Overview

CellenONE® is an open platform for isolating single cells and dispensing nanoliters.

cellenONE® X1

Cell morphology and/or multichannel fluorescence can be used to sort and dispense single cells using the standalone cellenONE instrument

cellenONE® X1 BSC

For applications requiring complete sample, operator, and environmental protection, the cellenONE instrument is integrated under a class II biosafety cabinet

Features

High accuracy single cell isolation and dispensing

  • CellenONE® is a ground-breaking platform that offers high-precision single cell isolation and dispensing in real time
  • It is based on sciDROP PICO precision dispensing technology and combined with advanced image processing
  • CellenONE® generates droplets using gentle acoustic waves, preserving exceptional cell viability for all cloning applications and maintaining protein expression for sequencing applications

CellenONE®

Image Credit: Cellenion

Versatile technology for a variety of samples

  • High-resolution optics make it possible to isolate any type of cell from any sample size, including mammalian cells, bacteria, fungi, archaea, and plants
  • Aspirate/dispense mode operation enables high recovery of a variety of samples, ranging in size from tiny cell suspensions with just a few microliters and a few dozen cells to much larger samples with thousands of cells

CellenONE®

Image Credit: Cellenion

Benefits

  • High accuracy single cell isolation and dispensing
  • Gentle on cells, ensuring outstanding cell viability
  • Compatible with a wide range of particle sizes (0.5 µm–80 µm)
  • Ideal for rare cell samples (zero dead volume and high recovery)
  • High-throughput isolation of hundreds of cells in only a few minutes
  • Selection of the cells of interest with user-defined sorting parameters (morphology and/or fluorescence markers)

CellenONE®

Image Credit: Cellenion

Applications

Single cell omics and cell line development applications can both benefit from cellenONE®’s low volume, high viability, and high accuracy single cell isolation systems

Single cell omics

CellenONE®

Image Credit: Cellenion

Cell line development 

CellenONE®

Image Credit: Cellenion

Cellenion has worked successfully with a large variety of cell types

  • Cell lines
    • CHO, Hybridoma, HEK293T, HeLa, A549, PC3, H1975, HepaRG
  • Nuclei
    • From cell line, fresh frozen (FF) and formalin-fixed paraffin-embedded (FFPE) tissue slices
  • Microbeads
    • PMMA microbeads from 2 to 30 µm diameters
    • TOYOPEARL®
    • PS microbeads
  • Primary cells
    • PBMC (including B- and T- cell fractions)
    • Fibroblasts, keratinocytes, melanocytes, cardiomyocytes, HUVEC
    • Neural stem cells

CellenONE®

Image Credit: Cellenion

Specifications

Source: Cellenion 

cellenONE®  X1
Sorting Technology Brightfield and/or
Multi-fluorescence images
(Blue, Green, Orange and Red)
Dispensing Technology sciDROP PICO:  150-600 pL / single drop
Number of Dispensing Channels Up to 2
Spottable Area (X x Y) 252 x 112 mm
Target Capacity 2 MTPs or 8 standard glass slides
Axis System X-Y Linear Magnetic Drive, Z Spindle Drive
Axis Precision < 3 µm
Dimensions with Enclosure (L x W x H) 650 x 700 x 1590 mm
Weight 205 kg

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