sciBUFFER

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Overview

Since 7.4 is the typical pH of human blood, many diagnostic assays are created at this level.

However, it is necessary to differentiate between coating or spotting buffers and assay buffers for solid phase assays, such as microarray applications. These two process steps have different buffer specifications.

Maximum immobilization effectiveness, uniform spot morphology, and upstream assay requirements are all met while the capture biomolecule is kept functional.

Moreover, it is not just a matter of pH; if the immobilization chemistry relies on the coupling of primary amines to functional groups on the surface, buffers with amines like Tris must be avoided. The involved biomolecules must function as well as possible in the assay buffer.

For instance, with a 10-fold dilution, the pH of a 100 mM sodium phosphate buffer rises from 6.7 to 6.9.

In most circumstances, a concentration of 25–50 mM is required to ensure a sufficient buffering capacity. The user could try a lower concentration, such as 10 mM, if the assay uses enzymes that are sensitive to high ionic strength.

sciBUFFER

Image Credit: Scienion

Applications

sciBUFFERs for DNA and Oligos

sciBUFFER

Image Credit: Scienion

Table 1. Source: Scienion

Product Name	Product Description	Contains	Product Number
sciSPOT cDNA	Printing buffer based on SSC (pH 7) with a detergent, to enable optimal microarray printing of cDNA samples.	50 mL 2x conc.	CBD-5411-50
sciSPOT Oligo B1	Printing buffer based on SSC (pH 7) and is optimized for use with oligonucleotides on functionalized glass slides.	50 mL 2x conc.	CBD-5421-50
sciSPOT Oligo B2	Printing buffer based on phosphate buffer (pH 9), for producing microarrays on polymer surfaces (sciPLEXPLATEs)	50 mL 2x conc.	CBD-5418-50
sciPROCESS-cDNA	Blocking buffers for post-printing treatment, used to deactivate the residual functional groups on the surface and minimize unspecific binding of the target DNA/RNA.	500 mL 2x conc.	CBD-5416-500
sciPROCESS-Oligo		500 mL 2x conc.	CBD-5426-500
sciPROCESS-Oligo		300 mL 2 x conc.	CBD-5426-300
sciHYB	Hybridization buffer to labeled cDNA or PCR products and immobilized capture probes, while minimizing cross-hybridization. Hybridization is accelerated and more efficient due to minimized secondary structures in probes and target sequences even at lower temperatures (42 °C).	1.5 mL 1x conc.	CBD-5417-1.5
sciHYB M1	Hybridization buffer, that, in contrast to standard sciHYB, consists of a non-toxic formulation and is used at more elevated temperatures (55-65 °C).	50 mL 1 x conc.	CBD-5419-50
sciWASH I DNA	Washing buffers to reduce background signal after the hybridization step. Contain a detergent with ionic strengths for different levels of stringency.	500 mL 5x conc.	CBD-5413-500
sciWASH II DNA		500 mL 5x conc.	CBD-5414-500
sciWASH III DNA		500 mL 5x conc.	CBD-5415-500
sciCOLOR T2	HRP substrate purpose-built for microarray applications	50 mL	CD-5600-50
		100 mL	CD-5600-100
		250 mL	CD-5600-250
sciCOLOR T3	Substrate purpose-built for black staining microarray applications	50 mL	CD-5603-50
		100 mL	CD-5603-100
		250 mL	CD-5603-250

sciBUFFERs for proteins

sciBUFFER

Image Credit: Scienion

Table 2. Source: Scienion

Product Name	Product Description	Contains	Product Number
sciSPOT Protein D1	Printing buffer based on PBS at pH 7.2. PBS is a nontoxic buffer that mimics the osmolarity and ion concentrations of the human body.	25 mL 2x conc.	CBP-5431-25
sciSPOT Protein D11	Printing buffer based on sciSPOT Protein D1, but contains an additive to slow down evaporation, which improves spot homogeneity.	25 mL 2x conc.	CBP-5435-25
sciSPOT Protein D12	Printing buffer based on sciSPOT Protein D1, but includes an additive to stabilize proteins, which helps maintaining nativity of the proteins and promotes shelf life of the microarrays.	25 mL 2x conc.	CBP-5436-25
sciSPOT Protein D2	Sodium Phosphate based buffer at pH 7.5.	25 mL 2x conc.	CBP-5439-25
sciSPOT Protein D3	Printing buffer based on TBS, pH 8.0.	25 mL 2x conc.	CBP-5441-25
sciSPOT Protein D4	Carbonate based printing buffer, pH 9.6	1.5 mL 1x conc.	CBP-5442-25
sciSTAB S3	Protein stabilizer that can be added to any of our sciSPOT buffers. This will enhance shelf life and functionality of your protein microarrays by stabilizing the native structure of the arrayed proteins.	2 mL 5 x conc.	CD-5601-2
sciBLOCK Protein D1	Designed for post-print processing of the protein microarrays and will passivate the surface. This PBS based buffer efficiently blocks unspecific binding, resulting in low background.	500 mL 5x conc.	CBP-5433-500
sciBLOCK Protein D1M		100 mL 1x conc.	CBP-5438-100
sciWASH Protein D1	PBS based washing buffer, suitable for all stages during multiplex ELISA.	500 mL 8x conc.	CBP-5432-500
sciWASH Protein D1M		250 mL 5x conc.	CBP-5437-250
sciBIND Protein D1	PBS based reagent diluent, which is ideally suited for dilution of your samples and secondary antibodies. This buffer is formulated to enhance your signals while reducing cross-reactivity.	1.6 mL 2x conc.	CBP-5434-1.6
sciBIND Protein D1		50 mL 2x conc.	CBP-5434-50
sciCOLOR T2	HRP substrate purpose-built for microarray applications	50 mL	CD-5600-50
		100 mL	CD-5600-100
		250 mL	CD-5600-250
sciCOLOR T3	Substrate purpose-built for black staining microarray applications	50 mL	CD-5603-50
		100 mL	CD-5603-100
		250 mL	CD-5603-250
sciSTAB S3	Protein stabilizer that can be added to any of our sciSPOT buffers. This will enhance shelf life and functionality of your protein microarrays by stabilizing the native structure of the arrayed proteins.	2 mL 5x conc.	CD-5601-2

sciBUFFERs SETs

Table 3. Source: Scienion

Product Name	Product Description	Contains	Product Number
sciBUFFERSET Oligo M1	Set of buffers for use with oligonucleotides on polymer surfaces	sciSPOT Oligo B2, sciPROCESS Oligo, sciHYB M1	CBP-5450
sciBUFFERSET Protein D1	Set of buffers for optimal microarray printing of protein samples	sciSPOT Protein D1, sciWASH Protein D1, sciBLOCK Protein D1, sciBIND Protein D1	CBP-5410
sciBUFFERSET Protein D1M	Set of buffers for optimal microarray printing of protein samples, up to 5 x96 well plates	sciSPOT Protein D1, sciWASH Protein D1M, sciBLOCK Protein D1M, sciBIND Protein D1M	CBP-5440

sciMULTIPLEX Box

Table 4. Source: Scienion

Product Name	Product Description	Contains	Product Number
sciMULTIPLEX Box	sciMULTIPLEX system contains selected consumables to facilitate transfer of multiple traditional singleplex immunoassays to one multiplex immunoassay. The intention of this kit is to expedite setting up a proof-of-principle assay as a solid base for further development and validation.	2x sciSOURCEPLATE 384 PP, 2 sciPLEXPLATE Type 1, 2x Type 2 and 2x solid black, sciSPOT Protein D1, D2, D3, D4 (5 mL each at 2 conc.), sciSTAB S3, sciBLOCK Protein D1M, 2x sciWASH Protein D1 (75 mL, 8x conc.) and sciCOLOR T2	CD-5602

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