Big Tuna: The ultimate automated buffer exchange solution

Buffer exchange and sample concentration are time-consuming processes and require a lot of manual work. The Big Tuna from Unchained Labs provides an automated solution for exchange to formulate, concentrate and clean up proteins or gene therapy vectors such as AAVs, VLPs and LNPs.

All these can be performed with just 30 minutes of setup time. The Big Tuna eliminates slow and manual methods of preparing samples to enable users to dedicate their time to other critical work.

It operates on many sample types, including:

  • LNP
  • mRNA and DNA
  • VLP
  • AAV
  • Protein

Unchained Tackles Science in 5 - Buffer Exchange with Big Tuna

 

Video Credit: Unchained Labs

Buffer exchange in 24 or 96 well formats

Big Tuna is capable of using two plate-based formats that enable scientists to exchange and concentrate 24 or 96 samples simultaneously. The system includes Unfilter 96 for users to exchange as little as 100 μL for up to 96 different samples in a run.

Unfilter 24 enables users to perform large exchanges of as much as 8 mL on up to 24 samples. A variety of molecular weight cutoff options are available to ensure that users have the right Unfilter for their experiment.

Big Tuna: The ultimate automated buffer exchange solution.

Image Credit: Unchained Labs

Hands-off, automated buffer exchange

Thanks to its exclusive pressure-based UF/DF process and gentle mixing, Big Tuna maintains buffer exchange evenly across the plate. The system's acoustic sensor is capable of measuring the volume in each well after each cycle to measure the variation in sample exchange rates. Big Tuna then calculates and adds back the amount of buffer needed to make it uniform before the next cycle. The process continues until it's reached the programmed exchange and concentration target.

Big Tuna: The ultimate automated buffer exchange solution.

Image Credit: Unchained Labs

Concentrate dilute samples

It is hard to handle dilute samples. The Big Tuna is capable of concentrating 48 mL down to 8 mL for up to 24 samples simultaneously. Users can follow up with buffer exchange to readily get the AAVs and other high-volume, low-concentration samples to where they are more easily accessible.

Big Tuna: The ultimate automated buffer exchange solution.

Image Credit: Unchained Labs

Exchange your samples exactly how you want

It is not necessary for buffer exchange to be a shot in the dark or to keep the user anchored to the bench throughout the process. The software wizard uses drag and drop for an operator to intuitively place the samples and buffers.

The system guides users through all the methods for customizing the run for a specific sample type. The simple setup allows users to have more control over the experiment with very little hands-on time.

Big Tuna: The ultimate automated buffer exchange solution.

Image Credit: Unchained Labs

Check in on progress

The Big Tuna is a reliable system for sample processing. The system walks users through the setup process before they walk away. A user check in on the progress of operations at any given time and see information regarding the time remaining for exchange and concentration runs. The system does not require manual monitoring and frees users to do other tasks.

Big Tuna: The ultimate automated buffer exchange solution.

Image Credit: Unchained Labs