Overview

The harmful effects of drugs and physical stressors on cells are measured through cytotoxicity assays. C.LIVE Tox reagents employ a straightforward mix-and-read procedure to assess cytotoxicity in real-time.

C.LIVE Tox

Image Credit: CYTENA GmbH

C.LIVE Tox Green and Red are both nonpermeable to living cells and nonfluorescent on the outside. The reagent produces intense fluorescence after attaching to DNA and passing through broken plasma membranes to penetrate dead cells. The addition of C.LIVE Tox reagents had no impact on the growth or morphology of healthy cells.

C.LIVE Tox

Image Credit: CYTENA GmbH

Advantages of CYTENA cytotoxicity assays

  • Simple mix-and-read workflow
  • Direct detection of dead cells with C.LIVE Tox reagents
  • Kinetic monitoring of cytotoxicity by measuring fluorescent objects over time
  • Designed for long-term live cell studies

Mix-and-read workflow

Perform the cytotoxicity assays in three easy steps: Plate the cells, add compounds using C.LIVE Tox reagent, and use the CELLCYTE X imaging system to kinetically evaluate cell health.

C.LIVE Tox

Image Credit: CYTENA GmbH

Real-time cytotoxicity analysis in the incubator

Without taking the cells out of the incubator, use the CELLCYTE X to image and analyze the fluorescent signals of the cytotoxicity assay.

C.LIVE Tox

Image Credit: CYTENA GmbH

C.LIVE Tox

HeLa cells were treated with increasing concentrations of Staurosporine. C.LIVE Tox Green was added to each well (250 nM) and time-lapse images were obtained with the CELLCYTE X. The control well shows minimal green signal compared to Staurosporine-treated well at 48 hours (left panel). Automated quantification shows the time- and dose-dependent toxicity effects of Staurosporine (right panel). Image Credit: CYTENA GmbH

Applications

  • Measure the toxic effects on cells from drugs and physical stressors
  • Kinetic monitoring of cytotoxicity

C.LIVE Tox reagents product information

Source: CYTENA GmbH

C.LIVE Tox

C.LIVE Tox

Image Credit: CYTENA GmbH

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