Overview

HUREL® Dog is a co-culture made up of non-parenchymal stromal cells and cryopreserved primary dog hepatocytes.

Example Metabolic Activity (nmoles/hr/106 cells). Source: Visikol Inc.

Substrate Enzyme Concentration (µM) Day 1 Day 4 Day 8
7-Ethoxycoumarin Phase I 25 0.07 0.13 0.22
7-Hydroxycoumarin Phase II 100 9.18 19.58 9.24
7-Hydroxycoumarin Phase III 100 2.5 6.9 4.11

 

The HUREL PlatinumHeps™ Media supplemented with 10% serum was used to plate cryopreserved primary dog hepatocytes, which were then switched to the HUREL PlatinumHeps™ Basal Media 24 hours after seeding.

The CYP substrate concentrations and metabolite formation, which is expressed as nmoles/hr/106 cells, are listed in the table above. Upon cell delivery on days D1, D4, and D8, all incubations were performed in triplicate and incubated for 60 minutes.

At 37 °C and with 5% CO2, reactions took place in a humid incubator. In preparation for further LC/MS/MS analysis, collected supernatants were kept at –20 °C.

  • Day 1-Morphology

HUREL® Dog™

Phase contrast image in a 96-well at a 10× magnification. Image Credit: Visikol Inc.

  • Day 7-Morphology

HUREL® Dog™

Phase contrast image in a 96-well at a 10× magnification. Image Credit: Visikol Inc.

  • Day 7-Bile Canaliculi

HUREL® Dog™

Bile canaliculi assayed via 5-(and-6)-carboxy-2’, 7’-dichlorofluorescein diacetate (CDFDA) stain at a concentration of 5 µM and imaged in the GFP channel in a 96-well at 10× magnification. Image Credit: Visikol Inc.

Example culture origin

Dog donor demographics

  • Strain: Beagle
  • Number of Donors: 1
  • Age (years): 5 months
  • Gender: Male

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