Overview

Cryopreserved primary Rabbit hepatocytes are co-cultured with non-parenchymal stromal cells in HUREL® Rabbit™.

Example Metabolic Activity (nmoles/hr/106 cells). Source: Visikol Inc.

Substrate Enzyme Concentration (µM) Day 1 Day 4 Day 8
7-Ethoxycoumarin Phase I (ECOD) 100 3.35 2.74 .94
7-Hydroxycoumarin Phase II (UGT) 100 295.33 278.50 265.33
7-Hydroxycoumarin Phase II(Sult) 100 48.17 37.83 30.65

 

Thawed and plated with 10% serum-supplemented HUREL PlatinumHeps™ Media, cryopreserved rabbit hepatocytes were switched to HUREL PlatinumHeps™ maintenance Media 24 hours after seeding.

The CYP substrate concentrations and metabolite formation, which is expressed as nmoles/hr/million cells, are listed in the table above. On Days 1, 4, and 8 (the customer day), all incubations were performed in triplicate and incubated for 60 minutes.

At 37 °C and with 5% CO2, reactions took place in a humid incubator. Before undergoing further LC/MS/MS analysis, collected supernatants were kept at –20 °C.

  • Day 1-Morphology

HUREL® Rabbit™

Phase contrast image in a 24-well at a 10× magnification. Image Credit: Visikol Inc.

  • Day 7-Morphology

HUREL® Rabbit™

Phase contrast image in a 24-well at a 10× magnification. Image Credit: Visikol Inc.

  • Day 7-Bile Canaliculi

HUREL® Rabbit™

Bile canaliculi assayed via 5-(and-6)-carboxy-2’, 7’-dichlorofluorescein diacetate (CDFDA) stain at a concentration of 5 µM and imaged in the GFP channel in a 24-well at 10× magnification. Image Credit: Visikol Inc.

Example culture origin

Animal donor demographics

  • Strain: New Zealand White
  • Number of Donors: 4
  • Age: –
  • Gender: Male

Other Equipment by this Supplier

While we only use edited and approved content for Azthena answers, it may on occasions provide incorrect responses. Please confirm any data provided with the related suppliers or authors. We do not provide medical advice, if you search for medical information you must always consult a medical professional before acting on any information provided.

Your questions, but not your email details will be shared with OpenAI and retained for 30 days in accordance with their privacy principles.

Please do not ask questions that use sensitive or confidential information.

Read the full Terms & Conditions.