Overview

Cryopreserved primary human hepatocytes are co-cultured with non-parenchymal stromal cells in HUREL® Human™.

Example Metabolic Activity (nmoles/hr/106 cells). Source: Visikol Inc.

Substrate Enzyme Concentration (µM) Day 1 Day 4 Day 8
Midazolam CYP3A4 5 0.18 0.25 0.13
Dextromethorphan CYP2D6 20 0.50 0.25 0.21
Tolbutamide CYP2C9 20 0.17 0.14 0.08

 

Human hepatocytes that had been frozen were thawed, plated on HUREL PlatinumHeps™ Media supplemented with 10% serum, and then switched to HUREL PlatinumHeps™ Basal Media 24 hours later.

The concentrations of CYP substrates are listed in the table above, and metabolite formation is expressed as nmoles/hr/106 cells. On Days 1, 4, and 8 after cell delivery, all incubations were performed in triplicate and incubated for 60 minutes.

The reactions happened in a humid incubator with 5% CO2 at 37 °C. Supernatants collected were kept at –20 °C until they underwent additional LC/MS/MS analysis.

  • Day 1-Morphology

HUREL® Human™

Phase contrast image in a 24-well at a 10× magnification. Image Credit: Visikol Inc.

  • Day 7-Morphology

HUREL® Human™

Phase contrast image in a 24-well at a 10× magnification. Image Credit: Visikol Inc.

  • Day 7-Bile Canaliculi

HUREL® Human™

Bile canaliculi assayed via 5-(and-6)-carboxy-2′, 7′ – dichlorofluorescein diacetate (C-DCFDA) stain at a concentration of 5 microns and imaged in the GFP channel in a 96-well at 10× magnification with filters EX/EM 492-495/512-527 nm. Image Credit: Visikol Inc.

Example culture origin

Human donor demographics

  • Gender: Male
  • Age (years): 65
  • Race: Caucasian
  • Cause of death: IHC-stroke
  • Drug history: Not given
  • Medications: Unknown statin, Lipitor, Lisinopril, Clonidine, and multivitamin
  • Medical History: Basel cell: 15–20 years ago. Removed with no radiation-received no chemo or radiation with no reoccurrence. Hypertension diagnosed 20–25 years ago

Donor serology

  • Hepatitis B: Negative
  • Hepatitis C: Negative
  • HIV: Negative
  • EBV: IgG [+], IgM [–}
  • RPR: Negative
  • CMV: Positive

Other Equipment by this Supplier

While we only use edited and approved content for Azthena answers, it may on occasions provide incorrect responses. Please confirm any data provided with the related suppliers or authors. We do not provide medical advice, if you search for medical information you must always consult a medical professional before acting on any information provided.

Your questions, but not your email details will be shared with OpenAI and retained for 30 days in accordance with their privacy principles.

Please do not ask questions that use sensitive or confidential information.

Read the full Terms & Conditions.