Overview

Co-culture made of cryopreserved primary rat hepatocytes cultured with cells of non-parenchymal, stromal type.

Source: Visikol, Inc.

Substrate Enzyme Concentration (µM) Day 1 Day 4 Day 8
7-Ethoxycoumarin Phase I(ECOD) C100 0.05 0.05 0.05
  Phase II(UGT)   0.05 0.05 0.05
  Phase II(SULT)   0.05 0.05 0.05
7-Hydroxycoumarin Phase II(UGT) 100 0.05 0.05 0.05
  Phase II(SULT)   0.05 0.05 0.05

 

Features

Culture conditions and morphology

Cryopreserved human hepatocytes were defrosted and plated with the help of the HUREL PlatinumHeps™ Media supplemented with 10% serum and consequently changed 24 hours post-seeding to the HUREL PlatinumHeps™ Basal Media.

In the table, CYP substrate concentrations are provided above in the table together with metabolite formation recorded as nmoles/hr/106 cells. All incubations were performed in triplicate on Days 1, 4, and 8 upon cell delivery and incubated for around 60 minutes.

Reactions occur in a humidified incubator ranging at 37 °C, in 5% CO2. Supernatants were gathered and stored at –20 °C, until the following LC/MS/MS analysis.

  • Day 1–Morphology

HUREL® Rat WH™

Phase contrast image in a 24-well at a 10× magnification. Image Credit: Visikol, Inc.

  • Day 7–Morphology

HUREL® Rat WH™

Phase contrast image in a 24-well at a 10× magnification. Image Credit: Visikol, Inc.

  • Day 7–Bile Canaliculi

HUREL® Rat WH™

Bile canaliculi assayed via 5-(and-6)-carboxy-2′, 7′ – dichlorofluorescein diacetate (C-DCFDA) stain at a concentration of 5 microns and imaged in the GFP channel in a 96-well at 10× magnification with filters EX/EM 492-495/512-527 nm. Image Credit: Visikol, Inc.

Example culture origin

Human donor demographics

  • Gender: Male
  • Age (years): 65
  • Race: Caucasian
  • Cause of death: IHC-Stroke
  • Drug history: Not provided
  • Medications: Unidentified statin, Lisinopril, Lipitor, Clonaidine, and Multivitamin
  • Medical history: Basel cell: 15 to 20 years ago. Eliminated with no radiation-received no chemo or radiation with no reoccurrence. Around 20 to 25 years ago, hypertension was diagnosed.

Donor serology

  • Hepatitis B: Negative
  • Hepatitis C: Negative
  • EBV: IgG [+], IgM [-}
  • HIV: Negative
  • CMV: Positive
  • RPR: Negative

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