Oris™ Cell Migration Assay

The Oris™ Cell Migration Assay from Platypus Technologies is a simple platform meant for publication-ready data.

The assay includes a 96-well plate with 'stopper' barriers that generate a central cell-free detection zone for cell migration experiments. When the stoppers are removed, the cells move to the detection zone at the center of every well.

Image Credit: Platypus Technologies LLC

Users can be Confident in Their Data

The Oris™ Cell Migration Assay kits offer reliable detection zones to provide users with excellent precision, accuracy and reproducibility in their experiments. The Oris™ Cell Migration Assay offers the perfect option for high quality, publication-ready data with fewer samples.

Image Credit: Platypus Technologies LLC

Rapid Experiment

The Oris™ Cell Migration Assay kits are compatible with all adherent cell lines, and the assay wells are covered with an extracellular matrix (Collagen I, Fibronectin, or Tissue Culture) to support the migration of the most common types of cells.

The Oris™ Cell Migration Assay can also be used with high content analyzers and plate readers, allowing users to quickly measure the migration of cells at any time during the course of their experiments.

Versatile to Suit Users’ Needs

The Oris™ Cell Migration Assay kits can be used to detect cell migration promoters and cell migration inhibitors. For scientists working in pharmaceutical firms and academic fields, the Oris™ Cell Migration Assay is a robust tool to progress studies in wound healing, drug discovery, or cancer research.

Single Plate Kits

Table 1. Source: Platypus Technologies LLC

Five Plate Kits

Table 2. Source: Platypus Technologies LLC

Maximum Flexibility

While the majority of the Oris™ Cell Migration Assay products already have the stoppers in place, stoppers are individually packaged in the Universal and FLEX kits:

• The Oris™ FLEX kit comes with four vacant plates along with four packs of 24 stoppers.
  • The FLEX kit can be used if users wish to examine fewer than 96 specimens for every experiment and still want to leverage the cost savings and expedited assay time provided by small wells.
• The Oris™ Universal Cell Migration Assembly Kits are supplied with a single empty plate for every pack of 96 stoppers. It comes in a 5-pack or 1-pack.
  • The Universal kit can be used if users wish to apply their own coating to the plate.

Table 3. Source: Platypus Technologies LLC

The Oris™ Cell Migration Assay is used in the following studies:

• Y. Lee et al. “Downregulation of PRMT1 promotes the senescence and migration of non-MYCN amplified neuroblastoma SK-N-SH cells” Sci Rep 9, 177; 2019.
• A. Kanda et al. “TGF-β-SNAIL axis induces Muller glial-mesenchymal transition in the pathogenesis of idiopathic epiretinal membrane” Sci Rep 9, 673; 2019.
• M. Gnanamony et al. “Chronic radiation exposure of neuroblastoma cells reduces nMYC copy number” Oncology Lett. 14(3); 2017.
• H. Marei et al. “Differential Rac1 signalling by guanine nucleotide exchange factors implicates FLII in regulating Rac1-driven cell migration” Nature Comm. 7; 10664; 2016.
• S. Ravi et al. “Incorporation of fibronectin to enhance cytocompatibility in multilayer elastin-like protein scaffolds for tissue engineering” J. Biomed Mater. Res. A 101(7); 2013.
• W. Gough et al. “A Quantitative, Facile, and High-Throughput Image-Based Cell Migration Method Is a Robust Alternative to Scratch Assay” J. Biomol. Screen. 16(2); pp.155-163; 2011.