BioVision Glutamine Colorimetric Assay Kit

Glutamine (Gln), one of the most abundant amino acids with an uncharged amide as a side chain, is produced through the condensation of glutamate and ammonia. Although Gln is categorized as a non-essential amino acid, it is essential in various biological processes such as cell growth, protein synthesis, and regulation of acid-balance in mammalian kidneys.

Gln is the main source of nitrogen in cells for the production of hexosamines and nucleotides. Diets rich in glutamine are beneficial for patients who suffer from severe burns, cancer, AIDS/HIV, and Crohn’s disease. The Glutamine Colorimetric Assay kit available from BioVision is an easy-to-use and sensitive assay that is capable of detecting the biologically relevant Gln concentrations in several tissues and fluids.

The assay works on the basis of hydrolysis of Glutamine to Glutamate, which generates a stable signal directly proportional to the quantity of Gln in the sample. The assay can even detect as low as 25 µM of Gln in different biological samples.


The goal of BioVision is to offer customers novel tools for expediting biological research and drug discovery. BioVision provides more than 8,000 products comprising the most extensive array of assay kits for main targets in Metabolic pathways.


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Source: BioVision Incorporated

Cat # + Size K556-100
Size 100 assays
Detection method Absorbance (450 nm)
Applications Measurement of Glutamine in various biological samples
Features and benefits
  • Convenient and rapid protocol
  • This kit can detect as little as 25 µM of Glutamine in a variety of biological samples
Kit components
  • Hydrolysis Buffer
  • Development Buffer
  • Hydrolysis Enzyme Mix (Lyophilized)
  • Development Enzyme Mix (Lyophilized)
  • Developer (Lyophilized)
  • Gln Standard (Lyophilized)
Storage conditions −20 °C
Shipping conditions Gel pack
Usage For research use only! Not for use in humans.