BioXolver for Accelerating Biostructural Research

The BioXolver instrument from Xenocs uses a small-angle X-ray scattering (SAXS) to gather structural data on biological samples in solution. This structural data comprises macromolecule envelope and related parameters, flexibility information, and protein interactions.

So far, SAXS measurements on biological samples have been limited to synchrotron facilities or low-throughput laboratory setups that require large amounts of samples.

Designed with unique features, Xenocs’ BioXolver instrument overcomes these limitations. It offers:

  • High-quality data
  • Fully automated multi-sample measurements and data analysis
  • Potential to work consistently with minimal volumes of samples; this ability is directly available in users’ laboratory, thus allowing them to reduce their growth cycle and expedite the bio-structural research

Discovering macromolecular shape and interactions

The BioXolver instrument uses small-angle X-ray scattering (SAXS) to obtain structural information on biological samples.

With the help of SAXS, the BioXolver instrument quantifies significant structural parameters of biological samples in solution. These parameters include:

  • Molecular weight
  • Degree of folding
  • Low-resolution 3D protein shape
  • Maximum intra-particle distance
  • Radius of gyration

Key features

The BioXolver instrument gains from Xenocs’ two decades of continuous research and development in SAXS and provides a special combination—an exclusive level of automation and the ability to analyze low scattering biological solutions.

Following are the key features of the BioXolver instrument:

  • Clean beam and beamstopfree technology ensures excellent data quality
  • High-intensity source and optics
  • In-line size-exclusion chromatography (SEC) is directly connected to SAXS and UV-VIS for analyzing complex samples
  • BioCube sample cell allows machine vision control and loading of 5 µL volumes
  • In-line pipetting robot for tubeless sample loading avoids contamination and sample loss

In-Lab SEC-SAXS, protein-protein interactions

The BioXolver instrument enables regular characterization of biological macromolecules in laboratory settings, thus offering quick feedback for crystallization studies, bio-structural investigations, and protein formulation.

Application examples include:

  • Protein-protein interactions to learn about aggregation
  • High-throughput 3D protein envelope
  • Comprehensive analysis of oligomers during protein fibrillation
  • In-lab SEC-SAXS for structural analysis of complex samples

Video Credit: Xenocs

High-quality SAXS data in the lab.

High-quality SAXS data in the lab. Image Credit: Xenocs

High-throughput measurements and results.

High-throughput measurements and results. Image Credit: Xenocs

Low sample volume consumption.

Low sample volume consumption. Image Credit: Xenocs