The Gator® Plus is a medium-throughput device that can be used for quantitation, kinetics, and epitope binning. This device provides speedy results using biolayer interferometry (BLI) technology.

Users can get more “hands-off” time and rise above the noise.

This fluidics-free device can accommodate microplates with 96 or 384 wells. Rich datasets can be obtained with a walk-away time of more than six hours and little hands-on effort. A variety of biomolecules, including small molecules, antibodies, and proteins, can be analyzed with Gator® Plus.

The enhanced baseline stability provided by Gator® Plus results in high-quality data that can be precisely analyzed for even the most challenging pairs. Quantifying pM affinity pairings with Gator® Plus is easier due to its better signal-to-noise ratio.


Table 1. Source: Gator Bio, Inc.

General Specifications Gator® Prime Gator® Plus Gator® Pro
Detection Technology Next-gen Biolayer Interferometry
Simultaneous Reads 8 8 8, 16, 24, and 32
Spectrometers 8 8 32
Acquisition Rate 2, 5, and 10 Hz
Temperature Control Ambient plus 4 °C to 40 °C
Dimension (HxWxD) and Weight 47 x 31 x 67 cm, 35 kg 68 x 44 x 73 cm, 55 kg 84 x 114 x 77 cm, 220 kg
Automation Compatible No No Yes
Performance Specifications Gator® Prime Gator® Plus Gator® Pro
Sample Types Proteins, antibodies, peptides, nucleic acids, liposomes, viruses, small molecules
Maximum Sample Capacity 168 456 1152
Types of Analysis Yes/no binding, quantitation, kinetics, affinity, off-rate ranking, epitope binning
Minimum Sample Volume 100 μL 40 μL 40 μL
Baseline Noise (RMS) ≤ 4 pm
Baseline Drift ≤ 0.12 nm/hour ≤ 0.1 nm/hour ≤ 0.1 nm/hour
Associate Rate kon 101 to 107 M-1 s-1
Dissociation Rate koff 10-6 to 10-1 s-1
Affinity Constant KD 10 pM to 1 mM
Binning Capacity 12x12 16x16 32x32


Gator® Plus: Quantitation, kinetics, and epitope binning through biolayer interferometry

Image Credit: Gator Bio, Inc.

Biolayer Interferometry (BLI)

Gator® systems are label-free analysis tools that use next-generation biolayer interferometry (BLI) technology. Biosensing probes are immersed in samples to identify biomolecular interactions.

Micro glass rods called Gator® probes have specific optical coatings and surface chemistries on their distal ends.

The association or disassociation of biomolecules causes a phase shift in the optical interference pattern produced by the sensing surface of a probe. Binding curves can be yielded through continuous phase-shift measurements.

Gator® Plus: Quantitation, kinetics, and epitope binning through biolayer interferometry

The sensorgram shows the real-time association and disassociation curves for a binding kinetics experiment using a Gator® system. Image Credit: Gator Bio, Inc.

The ease of use, adaptability, flexibility, and throughput of Gator® systems have facilitated numerous applications in manufacturing, life science research, and pharmaceutical development.

A Powerful Tool for Discovery, Development, and Manufacturing

In comparison to other commercial BLI products, the next-generation BLI is more sensitive and offers more robust performance. Various applications, from drug discovery to therapeutic manufacturing, are also supported

  • Biotherapeutics
    • Kinetics analysis
    • Epitope binning
    • Manufacturing QC
    • Process development
    • Pharmacokinetics
    • Antibody titer measurements
  • Gene therapy
    • Neutralizing/total antibody detection
    • AAV quantitation and kinetics
    • Receptor interaction
    • Gene expression
  • Drug discovery and development
    • Protein–small molecule interaction
    • Peptide binding analysis
  • Life science research
    • Receptor–ligand binding
    • Protein-protein interaction
    • Assay development and optimization

A User-Friendly Label-Free Technology

The components of Gator® Systems include instruments, probes, and a software program for data collection and processing.

  • Assay template generation
  • Real-time binding curves
  • Epitope binning
  • Report generation
  • Simple and fast assay setup
  • Kinetics and affinity analysis
  • Automated quantitation
  • Quantitation, kinetics, and regeneration in one run

Gator® software for GMP and GLP

Gator® Part11 Software enables users in GMP or GLP environments to adhere to FDA 21 CFR Part 11 standards. The data that the Part11 Software gathers is time-stamped and traceable. Account management, improved audit trails, and documented user sessions are all in accordance with FDA guidelines.

A Full Suite of Applications

Table 2. Source: Gator Bio, Inc.

Gator® Probe Function Application Dynamic Range Regeneration
ProA IgG titer Q 0.02 – 2000 μg/mL Yes
ProG IgG titer Q 0.02 – 2000 μg/mL Yes
ProL Kappa light chain titer Q 0.02 – 2000 μg/mL Yes
SA Biotinylated and Avi-tagged molecules K/EP Protein dependent No
SA XT Biotinylated proteins and large molecules K Protein dependent No
Flex SA Kit Reusable SA kit K Protein dependent Yes
SMAP Measurement of small molecules, peptides (<150 Da) K Protein dependent No
HFC Human IgG characterization Q/K/QKR/EP 0.05 – 300 μg/mL Yes
HFCII Advanced human IgG characterization Q/K/QKR/EP 0.3 – 6000 μg/mL Yes
MFC Mouse IgG characterization Q/K/QKR/EP 0.02 – 2000 μg/mL Yes
Anti–FAB F(ab), F(ab’)2 Q/K/QKR/EP 0.3 – 3000 μg/mL Yes
APS Direct adsorption K Protein dependent No
AR Amine coupling immobilization K/EP Protein dependent No
His His-tagged proteins Q/K/QKR/EP Protein dependent Yes
Ni-NTA Kit His-tagged proteins through Ni-NTA Q/K/QKR/EP 0.25 – 1000 μg/mL Yes
AAVX Direct binding titer (AAV1-10) Q/K 1x109 – 1x1013 vp/mL Yes
AAV9 Direct binding titer (AAV9) Q/K 3x109 – 1x1013 vp/mL Yes
HS AAV Kit High sensitivity titer (AAV1-8, 10) Q 1x107- 5x1010 vp/mL No
HS AAV9 Kit High sensitivity titer (AAV9) Q 1x107 - 1x109 vp/mL No
AAV Ratio Kit Empty vs Full Ratio Determination Ratio 0-100% full No
Anti-Rabbit Rabbit-IgG Q/K 1 - 500 μg/mL Yes
Anti-FLAG FLAG-tagged proteins K Protein dependent No
SARS-RBD RBD antibodies and receptors Q Protein dependent Yes