Defined & Controllable Extracellular Matrix for Cell Culture

Biochemically defined and animal-free - Mussel Adhesive Protein based matrix (MAPtrix™) from AMSBIO provides a highly defined and controllable recombinant extracellular matrix (ECM) for cell culture, which can be tailored to the specific needs of particular cell types.

MCF10A mammary epithelial cells forming an aggregate in MAPtrix™ HyGel. The thick section is about 100 μm. This image demonstrates a potential for morphogenesis, differentiation in basal / myoepithelial cells (CD49f -green) and luminal cells (muc1 - red), nuclei are labeled with DAPI. Image courtesy of Pierre Savagner, Institute of Cancer Research of Montpellier (France).

Low cost and ready-to-use - MAPtrix™ produces a reliable and reproducible protein coating on a wide variety of surfaces including glass, plastic and biological surfaces. Used as a replacement for traditional basement membrane extracts in drug delivery, surface-modified medical device, stem cell and tissue engineering scaffold applications - MAPtrix™ delivers superior cell plating efficiency, improved cell morphology and enhanced cell proliferation.

Mussel Adhesive Protein is a highly desirable substrate for use in a variety of biological and medical applications because of its strong wet adhesive, non-toxic, biodegradable and low immunogenic properties.

MAPtrix™ technology works by incorporating small individual motifs from ECM proteins into recombinant proteins with a backbone of Mussel Adhesive Protein to attach to the substrate, thereby presenting the ECM motif for cultured cells to attach to. Incorporating mix-and-match genetically incorporated bioactive peptides - MAPtrix™ provides an in-vivo-like surface structure, that mimics the native extracellular environment, enabling the maintenance of cells under serum and feeder-free conditions.

AMSBIO also offer MAPtrix™ Screen arrays to help scientists to screen a range of ECM motifs to find which ones their cells will adhere to. They can then use these motifs to tailor a defined microenvironment in 2D, or combine the MAPtrix biomimetic with a Polyethylene glycol (PEG) crosslinker to form a hydrogel (MAPtrix™ Hygel) for 3D cell culture.

For further information on MAPtrix™ animal-free recombinant ECM, please visit https://www.amsbio.com/maptrix-recombinant-ecm/ or contact AMSBIO on+44-1235-828200 / +1-617-945-5033 / [email protected].

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