Overview

A key enabling technology in the field of 3D bioprinting, Nanoscribe’s Two-Photon Polymerization (2PP) technology provides the highest resolution for 3D microfabrication on the scale of biological tissues and cells. To advance new developments in biology and the life sciences, it is necessary to use biomaterials compatible with 2PP technology.

The world’s leading authority on the synthesis of biomaterials, BIO INX creates materials for 3D bioprinting.

Three cutting-edge bioresins that are specially tailored for Nanoscribe’s Photonic Professional systems are included in the lineup of BIO INX Photoresins. Direct use of BIO INX bioresins increases batch-to-batch reproducibility of experiments while saving time and money that would have been spent by researchers making their own bioresins.

Nanoscribe users are better able to move their research from the lab to clinical trials by lowering these typical bioprinting obstacles.

You had to add inorganic additives to our photoresins to obtain functional or responsive materials. With Hydrobio INX N400 our customers now have a proven product for encapsulating cells as living ingredients for 4D materials.

Dr Remmi Baker-Sediako, Life Sciences Business Development Manager, Nanoscribe

Application areas

Hydrobio INX N400

  • The newest BIO INX Photoresin, Hydrobio INX N400, is a natural gelatin-based hydrogel created for live cell encapsulation to enable live cell printing
  • While maintaining the highest resolution to achieve feature sizes as small as 1 micron, the material properties of Hydrobio INX N400 have been customized to enhance naturally occurring cellular behaviors outside of the body
  • Researchers have more creative freedom when designing because of the material’s flexibility, which allows for cell encapsulation, cell seeding, and a combined strategy that uses both techniques
  • Researchers can now encapsulate cells, allowing them to be used as building blocks for living material systems like tissues rather than being restricted to just cell seeding

Advantages

  • Biofunctionalization: Supports cell encapsulation prior to printing for live cell printing
  • Biocompatibility: Supports cell adhesion and proliferation
  • Biodegradability: Enables cells to replace the extracellular matrix with tissue
  • Cell viability: 75% after one week
  • Easy handling: Presented in a ready-to-use kit that includes a concentrated stock solution, dilution buffer, and crosslinker for 10 prints in the presence or absence of cells
  • Direct use: Ready for printing in 10 minutes
  • Photodegradability: Enables multiphoton-induced photocleavage after printing

Possible applications

  • Tissue engineering: Fabrication of soft tissues that resemble the natural extracellular matrix.
  • Live cell printing: Human fibroblasts encapsulation
  • Cell seeding: 3D culturing of human-derived cell lines for cellular implants
  • Brain: Culture patient-derived glioma cells for organ-on-a-chips
  • Bone: Co-culturing of preosteoblasts and macrophages on 3D patterned surfaces
  • Breast: Cell invasion studies of human breast cancer cells on 3D scaffolds

Hydrotech INX N100

  • A synthetic, durable hydrogel called Hydrotech INX N100 is intended for the 3D microfabrication of intricate structures for tissue engineering applications
  • The material is both biologically inert and non-biodegradable. It can be coated, though, to promote cell adhesion and growth

Advantages

  • Processability: Easy processing into open and complex architectures with minimal deformation
  • Mechanical integrity: Very robust hydrogel suitable for stiff tissue engineering applications
  • Biocompatibility: Biocompatible with no toxic effect on living cells
  • Stability: Forms a biostable hydrogel that sustains 3D cellular structures. Suitable for long-term applications

Possible applications

  • Liver: HEPG2 viability testing
  • Tissue engineering and life science applications that require the soft properties of a hydrogel
  • Skin: Culturing of connective tissue (i.e., fibroblasts)
  • Breasts: Culturing of adipose tissue-derived stem cells

Degrad INX N100

  • A biodegradable polyester-based resin called Degrad INX N100 is created for the fabrication of intricate 3D structures for tissue engineering applications
  • The biomaterial, the first commercially available biodegradable photoresin for 2PP applications, is extremely flexible
  • Biochemically, Degrad INX N100 is inert. It can be coated, though, to promote cell adhesion and growth

Advantages

  • Biocompatibility: Exceptional biocompatibility (ISO 10993-5) with no toxic effect on living cells
  • High resolution: Highest resolution ever reported for a biodegradable material (<500 nm)
  • Flexibility: Can generate strong yet flexible structures that are favorable for easy handling and processing
  • Biodegradability: Degradable in a long term (3–5 years) when in contact with water or biological fluids
  • Processability: Easy processing into open and complex architectures with minimal deformation

Possible applications

  • Tissue engineering and life science applications requiring biomaterials with high mechanical stability and flexibility
  • Bone and cartilage regeneration
  • Connective tissue: No cytotoxicity for fibroblasts

Specs

Source: Nanoscribe 

  Hydrobio INX N400 Hydrotech INX N100 Degrad INX N100
Material class Natural hydrogel Synthetic hydrogel Polyester
Bio-degradable Yes No Yes
Printable feature size ≥ 1 µm ≤ 1 µm ≤ 1 µm
Mechanical properties Storage modulus
G’ = 2 – 18 kPa
Young’s modulus
E = 30 – 40 MPa
Young’s modulus
E = 50 – 60 MPa
Swelling (vol.) 50 – 250 % n./a. n./a.

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