The Alpine® is the world’s first direct detection camera from Gatan to democratize cryo-EM.
Advantages
Alpine is a pioneering direct detection camera that revolutionizes cryo-electron microscopy (cryo-EM) accessibility.
By facilitating the capture of high-resolution structures on 100 – 200 keV microscopes, Alpine enables routine cryo-EM research on more economical, widely used instruments, thus broadening its utility within the research community.
- Achieves a detective quantum efficiency (DQE) that surpasses scintillator-based cameras by up to 2.4 times.
- Regularly acquires high-resolution data on 100 – 200 keV microscopes, transforming screening instruments into reliable data collection tools.
- Enables 2D classification of small molecules and enhances macromolecule reconstructions.
- Optimizes workflow efficiency and identifies issues early, leading to faster production of superior structures.
- Real-time counting ensures a seamless balance between data collection speed and quality, eliminating compromises.
![Transthyretin resolved to 4.6 Å at 100 keV.](https://d1g9yur4m4naub.cloudfront.net/images/equipments/ImageForEquipment_589_16977873398309480.png)
Transthyretin resolved to 4.6 Å at 100 keV. Transthyretin (55 kDa) resolved to 4.6 Å at 100 keV acquired with an Alpine camera on a Talos F200C (equipped with an SFEG) using CDS at ~5.2 e-/pixel/s. The study used a Gatan model 626 side-entry holder for all samples and Leginon for data acquisition. Data courtesy Gabe Lander, The Scripps Research Institute.
![Throughput versus total dose comparison.](https://d1g9yur4m4naub.cloudfront.net/images/equipments/ImageForEquipment_589_16977873584786065.png)
Throughput versus total dose comparison. When combined with Latitude software, Alpine delivers high-quality data at the highest imaging throughputs from 100 – 200 keV. Image Credit: Gatan, Inc.
![Aldolase resolved to 3.07 Å at 100 keV.](https://d1g9yur4m4naub.cloudfront.net/images/equipments/ImageForEquipment_589_16977873780249860.png)
Aldolase resolved to 3.07 Å at 100 keV. Aldolase (100 kDa) resolved to 3.07 Å at 100 keV acquired with an Alpine camera on a Talos F200C (equipped with an SFEG) at 45kx (0.851 Å/pixel) using CDS at ~5.2 e-/pixel/s, 100 ms frames, 10 frames/s. Samples were prepared by manual plunging samples onto glow discharged Quantifoil UltrAuFoil grids with 1.2 μm holes spaced 1.3 μm apart. The study used a Gatan model 626 side-entry holder for all samples and Leginon for data acquisition. Data courtesy Gabe Lander, The Scripps Research Institute.
![Apoferritin resolved to 2.7 Å at 100 keV.](https://d1g9yur4m4naub.cloudfront.net/images/equipments/ImageForEquipment_589_1697787397053535.png)
Apoferritin resolved to 2.7 Å at 100 keV. Apoferritin (550 kDa) resolved to 2.7 Å at 100 keV acquired with an Alpine camera on a Talos F200C (equipped with an SFEG) at 45kx (0.851 Å/pixel) using CDS at ~5.2 e-/pixel/s, 100 ms frames, 10 frames/s. Samples were prepared by manual plunging samples onto glow discharged Quantifoil UltrAuFoil grids with 1.2 μm holes spaced 1.3 μm apart. The study used a Gatan model 626 side-entry holder for all samples and Leginon for data acquisition. Data courtesy Gabe Lander, The Scripps Research Institute.