Overview
CellenONE® X1 is used for gentle and precise image-based single cell sorting.
It is tailored for:
- Single cell omics (any omics, omni- omics)
- Cell line development (any cell, any standard or custom labware)
- Precision low-volume dispensing (pL–nL range) for miniaturized sample preparation
Image Credit: Cellenion
Features
- Direct visual inspection of the sample
- Processing of any sample (from 1 µL and just a few cells)
- Image-based single cell selection (brightfield and/or 4-channel fluorescence)
- Full image record of isolated cells/clones
- Best-in-class monoclonal outgrowth rate
- Environmental controls (temperature, humidity)
- Up to 100% single cells
- Post-isolation monoclonality verification by the operator
Applications
- scRNA-seq
- scWGS
- scMS
- scATAC-seq
- mAb
- Stem cells
- CRISPR/CAS9
- Cloning
How it works
Image Credit: Cellenion
- Cell suspension is loaded in a glass capillary
- Capillary tip is placed in front of an optical system
- Capillary tip is segmented into two zones
- Ejection zone: volume corresponding to the next generated droplet
- Sedimentation zone: safety zone considering possible cell sedimentation
- The subsequent droplet is dispensed into the intended labware if it only contains a single cell that satisfies the user-specified requirements (size, shape, and fluorescence markers). In the absence of that, it is dispensed into a recovery vial to enable reprocessing
Specifications
Source: Cellenion
| cellenONE® X1 |
| Sorting Technology |
Brightfield and/or
Multi-fluorescence images
(Blue, Green, Orange and Red) |
| Dispensing Technology |
sciDROP PICO: 150-600 pL / single drop |
| Number of Dispensing Channels |
Up to 2 |
| Spottable Area (X x Y) |
252 x 112 mm |
| Target Capacity |
2 MTPs or 8 standard glass slides |
| Axis System |
X-Y Linear Magnetic Drive, Z Spindle Drive |
| Axis Precision |
< 3 µm |
| Dimensions with Enclosure (L x W x H) |
650 x 700 x 1590 mm |
| Weight |
205 kg |