CellenONE® X1

Overview

CellenONE^® X1 is used for gentle and precise image-based single cell sorting.

It is tailored for:

• Single cell omics (any omics, omni- omics)
• Cell line development (any cell, any standard or custom labware)
• Precision low-volume dispensing (pL–nL range) for miniaturized sample preparation

Image Credit: Cellenion

Features

• Direct visual inspection of the sample
• Processing of any sample (from 1 µL and just a few cells)
• Image-based single cell selection (brightfield and/or 4-channel fluorescence)
• Full image record of isolated cells/clones
• Best-in-class monoclonal outgrowth rate
• Environmental controls (temperature, humidity)
• Up to 100% single cells
• Post-isolation monoclonality verification by the operator

Applications

• scRNA-seq
• scWGS
• scMS
• scATAC-seq
• mAb
• Stem cells
• CRISPR/CAS9
• Cloning

How it works

Image Credit: Cellenion

• Cell suspension is loaded in a glass capillary
• Capillary tip is placed in front of an optical system
• Capillary tip is segmented into two zones
  • Ejection zone: volume corresponding to the next generated droplet
  • Sedimentation zone: safety zone considering possible cell sedimentation

• The subsequent droplet is dispensed into the intended labware if it only contains a single cell that satisfies the user-specified requirements (size, shape, and fluorescence markers). In the absence of that, it is dispensed into a recovery vial to enable reprocessing

Specifications

Source: Cellenion