To date, the freezing medium Bambanker™ has been used to cryopreserve over 120 cell types, each supported by peer-reviewed studies. They have now been compiled in one place for easy access and cross-referencing.
Cryopreservation is key when managing a workflow in the contemporary laboratory. It’s a technique that allows researchers to, in effect, ‘pause’ cellular activity while preserving key phenotypes, which is achieved by pairing a cryoprotectant with controlled cooling and ultra-low-temperature storage. Experiments can then be resumed at a later time with confidence that experimental continuity is maintained.
With mounting pressure to improve study reproducibility and an increasing demand to store and share well-defined cells (from standard cell lines to more advanced programs), many researchers are making it a priority to implement standardized, publication-supported freezing conditions. To verify these conditions, a DOI-linked table provides an overview of how the cell types cryopreserved using Bambanker™ are linked to the reports in peer-reviewed studies.
Cell Type Matters
Cell type directly influences the cryopreservation process. Depending on the type of cells, freezing stress can have a significantly varied impact. Key factors of influence include membrane composition, cell size, cytoplasmic water content, and sensitivity to osmotic shifts, each of which affects how a cell responds to cryoprotectants, cooling rates, and thawing conditions.
Sturdier immortalized cell lines typically demonstrate reliable recovery rates across a range of parameters. Conversely, primary cells and stem cells (such as induced pluripotent stem cells, or iPSCs) can be otherwise unforgiving when it comes to recovery: Minor variations in handling time, cell density, or post-thaw recovery conditions can seriously impact viability, attachment, growth kinetics, and even phenotype.
This is why there is value in accessing and cross-referencing examples that are both cell-type-specific and supported by the literature. They mitigate the need for trial and error by providing clear insights that describe how corresponding cells have been cryopreserved and recovered in practice.
Common Cryopreservation Pitfalls (And Why Results Can Vary)
Even the slightest variation in handling cell types within the same genus can yield considerably different outcomes after thawing, especially for more sensitive primary cells and stem cells.
Cell variation is commonly caused by freezing stress or over-confluent cultures, while other typical sources of variability include inconsistent cell density, fluctuations in cooling consistency, prolonged exposure to room temperature after adding cryoprotectant, and post-thaw recovery steps, such as dilution timing and initial culture conditions.
Having the capacity to standardize any common source of variability reduces variability across batches and improves comparability across experiments. This is why having direct access to published examples for reference makes a significant difference, as variables can differ from one lab to another, and even between closely related cell types.
Easy access to reliable referencing offers a fundamental starting point, mitigates uncertainty, and offers users a clear sense of what is likely to work under comparable conditions.
The DOI-linked article below presents peer-reviewed reports on cell types cryopreserved with Bambanker™ freezing medium. These insights give researchers and laboratories the power to quickly locate the relevant reference and see how others have addressed similar workflows.
Image Credit: Holo Art/Shutterstock.com
A Simplified Approach
Bambanker™ is a ready-to-use cryoprotectant developed for advancing mammalian cell freezing processes as it:
- Decreases the number of manual steps required
- Eliminates serum requirements
- Standardizes freezing conditions
- Supports reproducible results across laboratories
The reliable performance of Bambanker™ has been reported in over 120 peer-reviewed publications, encompassing a wide range of cell and sample types.
What the Full List Includes
- A curated table of over 120 cell types and samples cryopreserved using Bambanker™
- Direct DOI links to the original peer-reviewed studies
- One representative publication per cell or sample type to keep the table concise and easy to read
Image Credit: NIPPON Genetics EUROPE GmbH
Update Policy
This table is reviewed and updated annually to include newly published data. The current version includes all relevant literature published up to December 2025.
About NIPPON Genetics EUROPE GmbH
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