Short-Wave Infrared Quantum Dots with Compact Sizes as Molecular Probes for Fluorescence Microscopy

A team of researchers at the University of Illinois published a paper this January in the journal American Chemical Society describing short-wave infrared emitters that were developed for use as molecular labels for microscopy applications, using synthesized semiconductor quantum dots.

Fluorescence Microscopy

Image Credit: Vshivkova/Shutterstock.com

What the team innovated will likely benefit future imaging of blood vessels in deep tissues due to its potential to generate high-contrast and high-resolution images.

Developing SWIR probes for fluorescence microscopy

The use of fluorescent probes has found many uses in biomedical sciences due to its robustness and reliability in the detection, imaging, and tracking of molecules within cells or tissues. Fluorescent probes have been used for many years to identify proteins in samples along with their conformational changes, also, they have been used to determine the location of these proteins in vivo, these functions have allowed fluorescent probes to be used in monitoring biological processes happening in real-time, in vivo.

Scientists have used fluorescent probes to detect human disease, screen populations to determine those at risk, diagnose disease, develop drugs, and evaluate treatment efficacy.

Recent years have seen significant advancements in the use of fluorescent probes, along with the technology and equipment that supports it.

These advancements have helped to rapidly progress methods in microscopic molecular imaging, such as super-resolution optical microscopy and single-molecule imaging that have become well-established in the field of cellular biology.

However, there is one major limitation to the use of fluorescent probes, and this is that cellular components often have an intrinsic fluorescence that can work to obscure the signals emitted by the probes.

Scientists have been working to develop a method to overcome this limitation. Recent research has focused on the development and exploration of nanomaterial-based fluorophores. Researchers are designing and synthesizing these new probes specifically to emit light at wavelengths that span two infrared spectral windows (the first near-infrared window, NIR-I, and the second near-infrared window, NIR-II). The latter of these is known as short-wave infrared (SWIR).

Recent research has seen the rapid expansion of SWIR-emitting nanomaterials. Of these, light-emitting semiconductor quantum dots (QDs) have become frequently used in research because of their advantages, such as broad wavelength tunability, and intense and stable emission that facilitates the tracking of individual molecules.

The team wanted to develop SWIR probes for use in fluorescence microscopy that overcame the limitations of conventional probes.

To do this, they continued to the work on light-emitting semiconductor quantum dots (QDs), creating a new class that produces continuous spectral tenability in the visible, NIR-I, and SWIR ranges.

The research

The focus of the research was to design and develop QDs. The team controlled the emission range by tuning the alloy composition of the QDs, which were constructed of HgxCd1–xSe alloy cores that were red-shifted toward the SWIR via epitaxial deposition of HgxCd1–xS shells.

They demonstrated that the resultant SWIR probes could be created in a consistently small and equal size, allowing for the analysis of their molecular labeling performance over a broad wavelength.

The QDs the team developed was shown to have a quantum yield in the SWIR and a compact size, as well as long-term stability in aqueous media. These characteristics of the synthesized QDs allow for a potentially diverse set of applications of the probes in fluorescence microscopy.

The established SWIR probes showed a significant enhancement of the signal-to-background ratio, meaning that they may be able to be used to create ultrasensitive molecular imaging, capable of detecting low-copy number analytes in biological samples.

Establishment of new SWIR probes

New SWIR probes for fluorescence microscopy were developed by the Illinois-based team. These probes overcome the issue of background fluorescence that currently limits the use of fluorescence microscopy methods.

The SWIR probes yield brightness and stability in aqueous media and can detect analyses present in low concentrations.

Journal references:

Hassan, M. and Klaunberg, B. (2004). Biomedical applications of fluorescence imaging in vivo. Comp Med, 54(6), pp.635-44. https://www.ncbi.nlm.nih.gov/pubmed/15679261

Jensen, E. (2012). Use of Fluorescent Probes: Their Effect on Cell Biology and Limitations. The Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology, 295(12), pp.2031-2036. https://anatomypubs.onlinelibrary.wiley.com/doi/full/10.1002/ar.22602

Sarkar, S., Le, P., Geng, J., Liu, Y., Han, Z., Zahid, M., Nall, D., Youn, Y., Selvin, P., and Smith, A. (2020). Short-Wave Infrared Quantum Dots with Compact Sizes as Molecular Probes for Fluorescence Microscopy. Journal of the American Chemical Society, 142(7), pp.3449-3462. https://pubs.acs.org/doi/10.1021/jacs.9b11567

Sarah Moore

Written by

Sarah Moore

After studying Psychology and then Neuroscience, Sarah quickly found her enjoyment for researching and writing research papers; turning to a passion to connect ideas with people through writing.

Citations

Please use one of the following formats to cite this article in your essay, paper or report:

  • APA

    Moore, Sarah. (2020, February 19). Short-Wave Infrared Quantum Dots with Compact Sizes as Molecular Probes for Fluorescence Microscopy. AZoLifeSciences. Retrieved on March 02, 2024 from https://www.azolifesciences.com/news/20200219/Short-Wave-Infrared-Quantum-Dots-with-Compact-Sizes-as-Molecular-Probes-for-Fluorescence-Microscopy.aspx.

  • MLA

    Moore, Sarah. "Short-Wave Infrared Quantum Dots with Compact Sizes as Molecular Probes for Fluorescence Microscopy". AZoLifeSciences. 02 March 2024. <https://www.azolifesciences.com/news/20200219/Short-Wave-Infrared-Quantum-Dots-with-Compact-Sizes-as-Molecular-Probes-for-Fluorescence-Microscopy.aspx>.

  • Chicago

    Moore, Sarah. "Short-Wave Infrared Quantum Dots with Compact Sizes as Molecular Probes for Fluorescence Microscopy". AZoLifeSciences. https://www.azolifesciences.com/news/20200219/Short-Wave-Infrared-Quantum-Dots-with-Compact-Sizes-as-Molecular-Probes-for-Fluorescence-Microscopy.aspx. (accessed March 02, 2024).

  • Harvard

    Moore, Sarah. 2020. Short-Wave Infrared Quantum Dots with Compact Sizes as Molecular Probes for Fluorescence Microscopy. AZoLifeSciences, viewed 02 March 2024, https://www.azolifesciences.com/news/20200219/Short-Wave-Infrared-Quantum-Dots-with-Compact-Sizes-as-Molecular-Probes-for-Fluorescence-Microscopy.aspx.

Comments

The opinions expressed here are the views of the writer and do not necessarily reflect the views and opinions of AZoLifeSciences.
Post a new comment
Post
Azthena logo

AZoM.com powered by Azthena AI

Your AI Assistant finding answers from trusted AZoM content

Your AI Powered Scientific Assistant

Hi, I'm Azthena, you can trust me to find commercial scientific answers from AZoNetwork.com.

A few things you need to know before we start. Please read and accept to continue.

  • Use of “Azthena” is subject to the terms and conditions of use as set out by OpenAI.
  • Content provided on any AZoNetwork sites are subject to the site Terms & Conditions and Privacy Policy.
  • Large Language Models can make mistakes. Consider checking important information.

Great. Ask your question.

While we only use edited and approved content for Azthena answers, it may on occasions provide incorrect responses. Please confirm any data provided with the related suppliers or authors. We do not provide medical advice, if you search for medical information you must always consult a medical professional before acting on any information provided.

Your questions, but not your email details will be shared with OpenAI and retained for 30 days in accordance with their privacy principles.

Please do not ask questions that use sensitive or confidential information.

Read the full Terms & Conditions.

You might also like...
Revolutionizing Fish Immunology: A Paradigm Shift in Antibody Responses