Novel Solution Revolutionizes Enzyme Production

The increasing need to switch to more environmentally friendly industrial processes is driving up demand for industrial enzymes. This research presents a new strategy for producing enzymes by using the P. pastoris platform’s cyanobacterial biomass, which has not yet been fully used.

Our work reveals the potential of cyanobacterial biorefineries to support enzyme production.”

Doris Schieder, Group Leader and Chair, Chemistry of Biogenic Resources, Technical University of Munich

This resulted from a comprehensive multidisciplinary approach. The team improved the efficiency of P. pastoris engineering by characterizing and growing a combinatorial library. Thorough screening of these libraries produced strong strains that produced enzymes, which made it possible to create an effective fed-batch method for AppA E. coli phytase production.

The effective use of Nostoc sp. De1 biomass hydrolysate as a fermentation substrate, which offers a practical and sustainable substitute for traditional feedstock, is a crucial component.

Our findings demonstrate that the extended P. pastoris toolkit not only generates high-performing producer strains but also offers a greener pathway for enzyme expression, addressing the critical need for sustainable biobased production.”

Korbinian Sinzinger, Research Assistant and Doctoral Candidate, Technical University of Munich