The BioXplorer 400P is an automated parallel pressure bioreactor system for innovative bioprocess discovery and optimization. The BioXplorer 400P is designed for applications ranging from syngas fermentation to cell-free enzymatically catalyzed processes. It employs pressure and precision gas management to accelerate bioprocesses that rely on the availability of dissolved gases.

The BioXplorer 400P’s flexible setup, paired with adaptable software, enables it to meet the particular demands of users, providing them with the data they require to scale up quicker.

BioXplorer 400P from H.E.L Group. Image Credit: H.E.L Group

Overview

Pressurized Bioprocessing

The BioXplorer 400P’s capacity to operate up to four concurrent bioprocesses at up to 10 bar pressure is unrivaled. More and more bioprocess scientists are turning to pressurized systems to improve the availability of dissolved gases and expedite gas-dependent bioprocesses.

The BioXplorer 400P is essential for optimizing and scaling up such operations. The system was designed with safety and convenience of use in mind. For example, using two gas spargers per reactor reduces the hazards of potentially dangerous gas combinations. Increasing the pressure on the bioprocesses can improve yields while preserving functionality and configurability.

Configurable System

The BioXplorer 400P has a modular architecture and can be outfitted with a variety of probes, gas feeds, and liquid feeds, allowing it to be adjusted to the user’s specific requirements. This maximizes functionality and ease of use while reducing bench space and cost.

Flexibility to Optimize Processes

The choice of probes to track the wide range of physicochemical parameters in bioprocess provides in-depth knowledge. The procedure can also be automated using H.E.L’s WinISO strict control and responsiveness. The BioXplorer 400P can run four bioreactors simultaneously or independently, minimizing the time required for bioprocess optimization.

Features and Options

Source: H.E.L Group

Specification Point BioXplorer 400P
Number of independent parallel bioreactors 4
Bioreactor material Glass body, stainless steel lid
Total volume 500 ml
Working volume 120 – 400 ml
Pressure rating Up to 10 bar
Sterilization Autoclavable bioreactors (including sensors)
Temperature Range 0 – 135 °C
Temperature resolution 0.1 °C
Stirrer drive Magnetically driven captive impeller or Overhead agitation (magnetically coupled)
Stirrer speed
  • Magnetically Driven – 250 – 1500 rpm
  • Overhead Agitation – 20- 2000 rpm
Auto re-couple
  • Magnetically Driven – Yes
  • Overhead Agitation – N/A
Liquid additions with independent control for each bioreactor
  • Magnetically Driven – 2 as standard, options for up to 2 additional liquid feeds
  • Overhead Agitation – 2 as standard, option for 1 additional liquid feed
Gas additions 1 set of 4 mass flow controllers allowing independent control for each reactor as standard, option for 2 additional sets
Standard options for MFC calibration Air, oxygen, nitrogen, hydrogen, carbon monoxide, carbon dioxide or methane
Gas spargers 2
Exhaust gas Peltier cooled condenser
pH control 1-way control (2-way control optional)
Polarographic DO sensing Optional
DO control Gas/liquid flow rate, stirrer speed (Optional)
BioVIS – Understand cell density trends without taking samples Optional
Foam/Level detection Optional
Control PC and software Windows® PC with WinISO control software preinstalled:
  • Fully independent control of all reactors
  • Advanced control strategies for stirring and liquid and gas additions based on feedback from sensors, time-based profiles, or event detection
  • Real-time display and logging of all reaction parameters
  • Easy export of data in a range of formats, including .csv

* Upper temperature limit for data acquisition from standard pH probes and optional ORP probes is 130 °C. The optional DO probe measures at temperatures above 135 °C. The lower temperature limit requires an integrated circulator option. 

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