Applying NMR to Study Small-Molecule Interactions in Drug Discovery

NMR is a powerful technique for uncovering the nature of small molecule interactions. This field has gained much interest in recent years, as the field of drug discovery and development has begun to heavily rely on the technique to elucidate ligand-sensing and disease-linked noncoding RNAs occurring naturally in the body that could have potential therapeutic use.

NMR

Image Credit: Smereka/Shutterstock.com

What is NMR?

Nuclear Magnetic Resonance (NMR) spectroscopy is a spectroscopic technique used in analytical chemistry to uncover the local magnetic fields around the atomic nuclei of a sample. This information can reveal the samples chemical composition and purity.

The technique involves establishing a magnetic field around the sample, generating an NMR signal via the excitation of the samples nuclei with radiofrequency waves, creating nuclear magnetic resonance. This is picked up by the sensitive radio receivers.

Atoms have an intramolecular magnetic field surrounding them. This has the impact of changing the resonance frequency.

These changes give insight into the electronic structure of the sample, giving details on its functional groups. Because the intramolecular magnetic fields are fairly unique to individual compounds, these signals can be used to determine the identity of a sample.

They can also be used to help biochemists identify proteins and other complex organic molecules.

Using NMR in the study of small-molecule interactions

NMR has several applications, particularly in research due to its ability to determine the identity of substances, as well as assessing its purity and defining its molecular structure. One area that has greatly benefited from exploiting the characteristics of this technique is drug research and development.

Many significant discoveries have been made via the use of NMR in drug discovery studies, and the number continues to grow. This has emerged out of using NMR to understand small molecule interactions to reveal naturally occurring ligand-sensing and disease-linked noncoding RNAs that may help develop new therapeutic compounds.

NMR has been used, since the 1970s, within the pharmaceutical industry to carry out studies into drug discovery.

Today, the number of papers using NMR in drug studies continues to increase, as the method becomes more popular, and more successes in the drug discovery field are gained as a result of running these kinds of tests.

A classical example of NMR application is the discovery of certain inhibitor and analysis of its binding mode, and a noteworthy one is the detection of allosteric regulator via NMR-based screening and its conformational analysis.

NMR has grown in popularity because of several advantages it has over other techniques. To begin with, NMR signals are produced both by chemical compounds and biomolecules. Also, the binding mode between them (for example, the tertiary structures, interaction interface, and conformational changes) can be determined at the atomic resolution. Finally, NMR can detect weak intermolecular interactions due to its high sensitivity.

NMR can be used in two ways in drug discovery research. The first is ligand-based. This is where proteins are detected via the ligands that they are bound to.

The approach which does not require isotope labeling is fast and produces sensitive NMR measurements. The method is also less impacted by contaminants and impurities but only on the condition that they do not intervene with the function and stability of the target protein and fragment.

However, there are limitations to the ligand-based approach. For example, it is difficult to apply to strong binders unless an advanced NMR technique is used. Also, because ligand signals cannot be used to determine the protein-bound form, the detection of target protein-ligand binding can be problematic.

The second method is the protein-based approach. Here, the heteronuclear single quantum correlation (HSQC) spectra of 1H-15N and 1H-13C are measured for uniformly 15N- and 13C-labeled proteins respectively. This process occurs in the absence or presence of ligands.

To identify the target proteins ligand-binding site, researchers look at the HSQC chemical shift perturbation and the ligand binding-induced signal broadening.

Even extremely low-affinity interactions can be detected with this approach, which is one of its benefits of use. In addition, it is simple to run, and preparations are inexpensive. Structural changes and ligand binding are easily monitored, which makes it a good approach for protein fingerprinting.

Structure-based drug discovery has greatly benefited from both these approaches, where the ligand-based approach has proved valuable in the screening of hit ligands, and the protein-based approach has proved useful in the process of hit validation and selecting hit ligands from both false-positive and non-specific binders.

NMR will likely continue to be used in drug discovery research. Already the number of pharmaceutical studies using NMR is growing, with numerous significant advancements being made in therapeutic development thanks to its use.

The future will likely see more NMR studies helping to push forward the pharmaceutical advances.

Sources:

Last Updated: Mar 20, 2020

Sarah Moore

Written by

Sarah Moore

After studying Psychology and then Neuroscience, Sarah quickly found her enjoyment for researching and writing research papers; turning to a passion to connect ideas with people through writing.

Citations

Please use one of the following formats to cite this article in your essay, paper or report:

  • APA

    Moore, Sarah. (2020, March 20). Applying NMR to Study Small-Molecule Interactions in Drug Discovery. AZoLifeSciences. Retrieved on April 20, 2021 from https://www.azolifesciences.com/article/Applying-NMR-to-Study-Small-Molecule-Interactions-in-Drug-Discovery.aspx.

  • MLA

    Moore, Sarah. "Applying NMR to Study Small-Molecule Interactions in Drug Discovery". AZoLifeSciences. 20 April 2021. <https://www.azolifesciences.com/article/Applying-NMR-to-Study-Small-Molecule-Interactions-in-Drug-Discovery.aspx>.

  • Chicago

    Moore, Sarah. "Applying NMR to Study Small-Molecule Interactions in Drug Discovery". AZoLifeSciences. https://www.azolifesciences.com/article/Applying-NMR-to-Study-Small-Molecule-Interactions-in-Drug-Discovery.aspx. (accessed April 20, 2021).

  • Harvard

    Moore, Sarah. 2020. Applying NMR to Study Small-Molecule Interactions in Drug Discovery. AZoLifeSciences, viewed 20 April 2021, https://www.azolifesciences.com/article/Applying-NMR-to-Study-Small-Molecule-Interactions-in-Drug-Discovery.aspx.

Comments

The opinions expressed here are the views of the writer and do not necessarily reflect the views and opinions of AZoLifeSciences.
You might also like... ×
Researchers identify a new compound for creating a new class of diabetes drugs