Researchers identify genes associated with autism spectrum disorder

The lack of some genes in the BEC/TCEAL cluster could be related to some alterations associated with the autism spectrum disorder, according to a preclinical study published in the journal Genome Biology, and led by Professor Jordi Garcia Fernàndez, from the Faculty of Biology and the Institute of Biomedicine of the University of Barcelona (IBUB), and researcher Jaime Carvajal, from the Andalusian Centre for Developmental Biology - University Pablo de Olavide (CSIC-UPO).

The study analysed transposon-derived genes that are involved in neural complex functions and that have not been studied before within the context of the autism spectrum disorder and other neurological diseases. The study, carried out with animal models, describes some molecular mechanisms that are determining in the development of the neocortex in humans and other placental mammals.

Among the authors of the study are the experts of the UB Enrique Navas, Bru Cormand, Gemma Marfany, Serena Mirra, Noelia Fernández-Castillo, Ester Antón and Carlos Herrera (also members of IBUB, el IRSJD and el CIBERER), and Eduardo Soriano and Fausto Ulloa (IBUB-CIBERNED-ICREA).

The study also counts on the participation of teams from the University Pablo de Olavide, the Centre for Genomic Regulation (CRG), Pompeu Fabra University, the University of Murcia, the Zoological Station Anton Dohrn in Naples (Italy) and Charles University (Czech Republic).

Transposon domestication: how can new functional genes originate?

The BEX/TCEAL cluster is a 14-gene genic family which is not much studied, and it is located in the chromosome X. This genic family codes small proteins from the hub proteinas (connected with many other proteins), which change their configuration according to the molecular context in which they are.

The article states that the genic group BEX/TCEAL resulted from a process known as transposon molecular domestication (genetic mobile elements that can be placed in different areas of the genome). Through this process, a non-functional transposon gen can become a new active element of the genome (domesticated transposon) which develops similarly to the other genes.

Transposons are regarded as a source of evolutionary innovation and adaptation in human beings.

These are genetic components that have no function or are harmful to the host genome. However, in the case of the BEX/TCEAL cluster, these were domesticated by the molecular machinery of the ancestor of placental mammals. That is, they became new genes!"

Jordi García-Fernández, Professor and Director, Department of Genetics, Microbiology and Statistics, UB

García-Fernández is also the head of the Research Group on Evolution and Development (Evo-Devo)

During the evolutionary process, transposons can lose their ability to jump "due to new mutations, which join the neighbouring effects of the regulator regions where they are, and transform these mobile elements into new genes that have not appeared before during the evolution", notes researcher Enrique Navas-Pérez (UB-IBUB), first author of the article.

According to researcher Jaime J. Carvajal, vice-rector of CABD and head of the Research Group on Molecular Embryology, "such events can have a great importance when setting unique characteristics of mammals. We are looking at the function of a series of genes that can have contributed to the establishment of specific brain properties of placental mammals".

Genes related to autism spectrum disorder

The study states that the BEX3 gene -an element in the BEX/TCEAL cluster-, plays a decisive role in the m-TOR path, a metabolic path related to proliferation and differentiation in many tissues, and specially those in the nervous system. In other studies, other genes from the cluster have been related to neurotrophins (molecules that regulate the neural proliferation in the embryonal nervous system) and p75, a receptor involved in neuronal death.

The conclusions show that the BEX3 gen -and probably other elements in the BEX/TCEA complex- could be implied in several aspects of the autism spectrum disorder and other neurological affectations.

Therefore, the mice that were affected by the lack of one of these genes, showed alterations in behaviour which are related to the autism spectrum disorder, apart from showing some anatomical and skeletal changes. "Mice without the BEX3 gene are antisocial, and do not interact with other mice", notes researcher Ángel Carrión, from the Department of Neurosciences of UPO.

"These are new genes, derived from transposons, which are involved in complex neural functions and which have not been studied until now within the context of the autism spectrum disorder and other neurological pathologies", note the researchers.

"Despite being young in evolutionary terms, they could integrate in established biological paths, becoming essential for the right functioning of the animal", notes Cristina Vicente-García, co-author of the article together with Enrique Navas-Pérez and Serena Mirra.

Authors reveal the level of expression of these genes in individuals with autism spectrum is low. As a result, researchers observed a wide range of manifestations in laboratory models, in particular, from autism to compulsive behaviours.

A decisive process in the evolution of placental mammals

Only a few genes in placental mammals are known -and specifically, no gene clusters- that derive from molecular domestication of transposons.

For instance, those associated with RAG1/2 proteins, which are key elements of the adaptive immune system of vertebrates, or syncytines, which enabled the development of the complex placenta. "Therefore, we think domestication of these transposons was an important process in the development of the neocortex in the group of placental mammals, to which we belong.

Therefore, the effect that generated the BEX/TCEAL cluster on the ancestral genome conditioned the development of the brain of placental mammals", note the researchers.

"There are still 14 new genes to study -which have not been practically studied until now-, which can be involved in the formation of the complex brain and several manifestations of the autism spectrum. Also, the relation virus-transposons-immune system is very intriguing. For instance, the composition of transposon families of the bat genome is exceptional among mammals, and bats are immune to many viral infections", concludes Jordi Garcia-Fernández.

Source:
Journal reference:

Navas-Pérez, E., et al. (2020) Characterization of an eutherian gene cluster generated after transposon domestication identifies Bex3 as relevant for advanced neurological functions. Genome Biology.  doi.org/10.1186/s13059-020-02172-3.

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