Cambridge researchers have discovered how T cells – an important component of our immune system – are able keep on killing as they hunt down and kill cancer cells, repeatedly reloading their toxic weapons.
Cytotoxic T cells are specialist white blood cells that are trained by our immune system to recognize and eliminate threats – including tumor cells and cells infected with invading viruses, such as SARS-CoV-2, which causes COVID-19. They are also at the heart of new immunotherapies that promise to transform cancer treatment.
Professor Gillian Griffiths from the Cambridge Institute for Medical Research, who led the research, said: "T cells are trained assassins that are sent on their deadly missions by the immune system. There are billions of them in our blood, each engaged in a ferocious and unrelenting battle to keep us healthy.
"Once a T cell has found its target, it binds to it and releases its toxic cargo. But what is particularly remarkable is that they are then able to go on to kill and kill again. Only now, thanks to state-of-the-art technologies, have we been able to find out how they reload their weapons."
Today, in a study published in Science, the team have shown that the refueling of T cells' toxic weapons is regulated by mitochondria. Mitochondria are often referred to as a cell's batteries as they provide the energy that power their function. However, in this case the mitochondria use an entirely different mechanism to ensure the killer T cells have sufficient 'ammunition' to destroy their targets.
These assassins need to replenish their toxic payload so that they can keep on killing without damaging the T cells themselves. This careful balancing act turns out to be regulated by the mitochondria in T cells, which set the pace of killing according to how quickly they themselves can manufacture proteins. This enables killer T cells to stay healthy and keep on killing under challenging conditions when a prolonged response is required."
Professor Gillian Griffiths, Cambridge Institute for Medical Research
To accompany the study, Professor Griffiths and colleagues have released footage showing killer T cells as they hunt down and eliminate cancer cells.
One teaspoon full of blood alone is believed to have around 5 million T cells, each measuring around 10 micrometers in length, about a tenth the width of a human hair. The cells, seen in the video as red or green amorphous 'blobs', move around rapidly, investigating their environment as they travel.
When a T cell finds an infected cell or, in the case of the film, a cancer cell, membrane protrusions rapidly explore the surface of the cell, checking for tell-tale signs that this is an uninvited guest. The T cell binds to the cancer cell and injects poisonous 'cytotoxin' proteins down special pathways called microtubules to the interface between the T cell and the cancer cell, before puncturing the surface of the cancer cell and delivering its deadly cargo.
The research was funded by Wellcome.
Lisci, M., et al. (2021) Mitochondrial translation is required for sustained killing by cytotoxic T cells. Science. doi.org/10.1126/science.abe9977.