Yale researchers have discovered a protein that helps protect hosts from infection with the tick-borne spirochete that causes Lyme Disease, a finding that may help diagnose and treat this infection, they report Nov. 11 in the journal PLOS Pathogens.
Lyme Disease is the most common vector-borne disease in North America and is transmitted by ticks infected with the spirochete Borrelia burgdorferi. The course of the disease varies among individuals, with the majority experiencing mild symptoms easily treated by antibiotics.
However, in some cases of untreated Lyme the infection can spread to the heart, joints, nervous system, and other organs.
For the study, the Yale team expressed more than 1,000 human genes in yeast and analyzed their interactions with 36 samples of B. burgdorferi. They found that one protein, Peptidoglycan Recognition Protein 1 (PGLYRP1), acts like an early warning signal to the immune system when exposed to the bacteria.
When exposed to the Lyme spirochete, mice lacking PGLYRP1 had much higher levels of B. burgdorferi than mice with the protein and showed signs of immune system dysfunction, the researchers report.
Stimulating the ability of people to make more of this protein could help fight infection."
Erol Fikrig, Study Corresponding Author, the Waldemar Von Zedtwitz Professor of Medicine (Infectious Diseases), Professor of Epidemiology (microbial diseases) and Microbial Pathogenesis
Fikrig and his colleagues are also investigating whether people with higher levels of PGLYRP1 may be less susceptible to infection by B. burgdorferi, which would help explain why some infected individuals have better outcomes.
Yale's Noah Palm and Aaron Ring are co-corresponding authors of the paper. Akash Gupta, Gunjun Arora, and Connor Rosen of Yale are co-lead authors.
The work was primarily funded by the National Institutes of Health and the Steven & Alexandra Cohen Foundation, a nonprofit that supports Lyme and tick-borne disease research.
Gupta, A., et al. (2020) A human secretome library screen reveals a role for Peptidoglycan Recognition Protein 1 in Lyme borreliosis. PLOS Pathogens. doi.org/10.1371/journal.ppat.1009030.