Overview
The F.SIGHT™ from Cytena enables single-cell dispensing. Dispensing is made simple with fluorescent sorting.
Features
Tailored for
- Single-cell omics
- Cell line development
- Low-volume assays (PCR, WGA, NGS setups)
Benefits
- Highly efficient
- Detection of low-copy or low-intensity dye numbers
- Comes with laser for red fluorescence (mCherry, RFP, Texas Red, Deep Red, and more)
Specials
- High-resolution objectives are available
- Consistent with several PCR plates
- Laser can be freely adjusted
- Combined plate de-ionizer removes electrostatic charge
Reliable and highly efficient single-cell dispensing
The F.SIGHT™ has the potential to dispense both fluorescent and unlabeled cells displaying the highest efficiency. The novel dual-camera system enables users to capture bright field and fluorescence information at complete resolution in a concurrent manner.
Image Credit: CYTENA GmbH
No electrostatic charge
Furthermore, 96 and 384 PCR well plates could be employed and with the plate chiller, users’ reagents will remain at the needed temperatures at the time of the deposition of the single cells. The fixed deionizer effectively eliminates electrostatic charge from users’ 96 or 384 well plates.
Deposited within a millimeter
The high-precision droplet deposition guarantees that each cell is deposited within millimeters. This allows the least amounts of pre-stored medium or reagents, which is perfect for single-cell omics and low-volume assays.
Visualization made easy
Cameras could be regulated separately and the built-in powerful blue laser has been adjustable in a free manner. Collectively, their high dynamic range enables users to envision even the feeble fluorophores.
Image Credit: CYTENA GmbH
Enabling greater insights
Fluorescent dyes can be used to label the products right in or on the cells producing them as F.SIGHT™ has the potential to visualize them, even when there are just a few of them.
Also, users can process non-fluorescent cells by just putting off the laser and making use of the F.SIGHT’s bright field sorting mode. Similar to that with its small brother—the C.SIGHT—cells can further be sorted with the help of morphological criteria.
No risk of bleaching or phototoxicity
Users can make use of their standard dyes like FITC, GFP, Calcein AM, CellTracker Green, and others. As a result of the non-transparent flap, their dyes and cells are safeguarded from ambient light at the time of the process.
Image Credit: CYTENA GmbH
Afraid of bleaching or phototoxic effects?
The laser beam simply illuminates the nozzle. The intensity of the laser can be easily changed. To keep the sample safe while using sensitive dyes, limit the laser intensity and increase the camera exposure duration instead.
Working with red fluorescence?
No issues, it is possible to equip their F.SIGHT with the availability of a laser for red fluorescence detection so that users can function with dyes like Texas Red, mCherry, RFP, Deep Red, and more.
Reliable and highly efficient single-cell dispensing
The availability of all-new software seems to be quick and intuitive. Users can design their separate fluorescence experiments in just minutes. Full-resolution bright field and fluorescence images separately plus an overlay image are being recorded and saved for additional analysis and proof of clonality.
Image Credit: CYTENA GmbH
Control the device from outside the cabinet
Users can just connect to the monitor, keyboard, and mouse or even make use of a remote control terminal.
Video Credit: CYTENA GmbH
Featured workflow
The wide product portfolio provides a range of ground-breaking technologies and instruments for considerably increasing the quality, speed, and efficiency of cell line development workflows.
Image Credit: CYTENA GmbH
Applications
- Deconvoluting clonal complexity of barcoded cell populations
- Fluorescence intensity-based isolation of single cells with assured clonality for CLD workflows
- Generation of CRISPR-edited mesenchymal stem cell clones for regenerative medicine applications
- Miniaturization and automation of a full-length single-cell RNA-seq workflow