Overview
Titer assays are used to determine the concentration of specific antibodies in samples (e.g., serum, plasma, and the culture supernatant). Fast and repeatable titer measurement is critical for identifying high producer cell lines for biotherapeutic production and facilitating high-throughput antibody development procedures.
The F.QUANT Titer assay kits, Powered by PAIA, are based on PAIA technology and are designed for fast measurement of antibody content in culture supernatant. The F.QUANT Fc Titer measures the concentration of Fc in IgG and other proteins that contain the Fc portion of IgG, like Fc fusion proteins and bispecifics. Human Fab fragments are recognized using the F.QUANT Fab Titer assay.
Image Credit: CYTENA GmbH
The F.QUANT Fc Titer test is used to evaluate increasing doses of Trastuzumab (0–2000 µg/ml). The fluorescence microplate reader was used to measure signal intensities.
Image Credit: CYTENA GmbH
Mix, shake, and read protocol for titer quantification assays
Follow these easy steps to conduct the titer quantification assays:
Shake the F.QUANT Titer Plate after adding the F.QUANT Titer Mix and supernatant, then read the plate using a fluorescence microplate reader.
Image Credit: CYTENA GmbH
Advantages of Cytena’s titer assays
- Direct titer quantification from the supernatant
- No cell removal or wash is necessary
- Easy to mix, shake, and read workflow
- Designed for clone screening in CLD
- Easy automation on liquid handlers
- Only 5 µL of the supernatant is required for assays
Kit contents
Each kit includes a 384-well F.QUANT Titer Plate consisting of desiccated capture beads and a bottle of fluorescent F.QUANT Titer Mix.
Features
- Excitation wavelength: 640 nm; Emission wavelength: 665 nm
- Fluorescence microplate reader
- Solution-based detection
Image Credit: CYTENA GmbH
F.QUANT Titer assay kits product information
Source: CYTENA GmbH
| PRODUCT NAME |
ID |
AMOUNT |
CONTENT |
STORAGE |
| F.QUANT Fc Titer |
CY.FQ.TIT.103 |
10 plates/3840 samples |
10x 384-well F.QUANT Fc Titer
Plate and 200 mL Fc Titer Mix |
4 °C |
| F.QUANT Fab Titer |
CY.FQ.TIT.104 |
10 plates/3840 samples |
10x 384-well F.QUANT Fab Titer
Plate and 200 mL Fab Titer Mix |
4 °C |
F.QUANT Fc Titer
*Trial size |
CY.FQ.TIT.101 |
2 plates/768 samples |
2x 384-well F.QUANT Fc Titer
Plate and 2x 25 mL Fc Titer Mix |
4 °C |
F.QUANT Fab Titer
*Trial size |
CY.FQ.TIT.102 |
2 plates/768 samples |
2x 384-well F.QUANT Fab Titer
Plate and 2x 25 mL Fab Titer Mix |
4 °C |
Applications
- Measuring the concentration of target antibodies
- Identifying high producer cell lines for manufacturing biotherapeutics
- Enabling high-throughput antibody discovery workflows