Overview

With this automated, logically designed workstation, users can optimize and streamline their entire cell line development (CLD) workflow. The brand-new C.STATION fully automates stable CLD for the production of monoclonal antibodies, from the single-cell cloning of transfected cells to the selection of high-producing clones to upscaling.

Features

C.STATION™

Image Credit: Cytena

  • The effective single-cell cloning and imaging device UP.SIGHT™ has a built-in titer quantification module
  • C.STUDIO software for clone selection, clone tracking, and creation of PDF reports for IND submissions
  • QINSTRUMENTS BioShake plate shaker
  • Static and suspension culture incubators with built-in shakers
  • The product comes with a “best-in-class” liquid handler with an 8-channel pipette head for plate filling, hit-picking, media exchange, fed-batch culture, and setting up screening assays
  • All of the components are contained in a small workstation with HEPA filters designed for sterile cell culture work

Application

C.STATION™

Image Credit: Cytena

  • The C.STATION incorporates the UP.SIGHT, the company’s signature tool, enables a probability of clonality >99.99% and meets regulatory requirements
  • Through nozzle images and 3D Full Well Imaging, a novel and cutting-edge technique, which images the full volume of each well from the point of dispensing using the integrated imager, clonality is double-assured
  • The dispensing nozzle is first imaged to ensure that only one cell is dispensed into each well and nonclonal droplets are discarded
  • 3D Full Well Imaging is used to confirm the single cells once more

Tracking colony growth over time

C.STATION™

Image Credit: Cytena

  • The UP.SIGHT easily tracks confluency, cell count, and viability of clonal cells
  • The C.STUDIO software allows users to follow the development of colonies throughout an experiment, and the instrument’s ability to replay and rewind images gives users a complete understanding of the CLD workflow
  • Choose the appropriate time point to select clones for a larger volume plate format using the confluency tool

Comprehensive mAb titer monitoring for high-producer identification

C.STATION™

Image Credit: Cytena

  • Utilizing a plate-based assay, the C.STATION has integrated titer quantification. Without involving the user, this enables the screening of hundreds of clones
  • Clone selection with the C.STUDIO is simple and ensures data traceability based on relative productivity

CLD automation has never been easier

  • Off-the-shelf validated and automated CLD workflow
  • Proven technology
  • Minimal hands-on time

Workflow

C.STATION™

Image Credit: Cytena

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C.STATION - Welcome to the future of CLD automation

Video Credit: Cytena