C.STATION™

Overview

With this automated, logically designed workstation, users can optimize and streamline their entire cell line development (CLD) workflow. The brand-new C.STATION fully automates stable CLD for the production of monoclonal antibodies, from the single-cell cloning of transfected cells to the selection of high-producing clones to upscaling.

Features

Image Credit: Cytena

• The effective single-cell cloning and imaging device UP.SIGHT™ has a built-in titer quantification module
• C.STUDIO software for clone selection, clone tracking, and creation of PDF reports for IND submissions
• QINSTRUMENTS BioShake plate shaker
• Static and suspension culture incubators with built-in shakers
• The product comes with a “best-in-class” liquid handler with an 8-channel pipette head for plate filling, hit-picking, media exchange, fed-batch culture, and setting up screening assays
• All of the components are contained in a small workstation with HEPA filters designed for sterile cell culture work

Application

Image Credit: Cytena

• The C.STATION incorporates the UP.SIGHT, the company’s signature tool, enables a probability of clonality >99.99% and meets regulatory requirements
• Through nozzle images and 3D Full Well Imaging, a novel and cutting-edge technique, which images the full volume of each well from the point of dispensing using the integrated imager, clonality is double-assured
• The dispensing nozzle is first imaged to ensure that only one cell is dispensed into each well and nonclonal droplets are discarded
• 3D Full Well Imaging is used to confirm the single cells once more

Tracking colony growth over time

Image Credit: Cytena

• The UP.SIGHT easily tracks confluency, cell count, and viability of clonal cells
• The C.STUDIO software allows users to follow the development of colonies throughout an experiment, and the instrument’s ability to replay and rewind images gives users a complete understanding of the CLD workflow
• Choose the appropriate time point to select clones for a larger volume plate format using the confluency tool

Comprehensive mAb titer monitoring for high-producer identification

Image Credit: Cytena

• Utilizing a plate-based assay, the C.STATION has integrated titer quantification. Without involving the user, this enables the screening of hundreds of clones
• Clone selection with the C.STUDIO is simple and ensures data traceability based on relative productivity

CLD automation has never been easier

• Off-the-shelf validated and automated CLD workflow
• Proven technology
• Minimal hands-on time

Workflow

Image Credit: Cytena

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Video Credit: Cytena