Overview
The B.SIGHT™ enables label-free single-cell isolation of bacteria and other microorganisms.
Dispense bacteria, yeast, and archaea using single-cell technology
It is possible to separate individual prokaryotic cells with the help of CYTENA’s validated single-cell dispensing technology. The B.SIGHT’s high-resolution optics make even the least cells visible. No staining or labeling is needed to separate a single bacterium.
Ideal for anaerobic conditions
The benchtop B.SIGHT could function in an anaerobic chamber as a result of its small footprint, low heat emission, and low particle emission. Such characteristics provide the ideal environment for isolating obligate anaerobes. Also, users can control the device from the exterior part of the cabinet by connecting a keyboard, monitor, and mouse, or by making use of a remote control terminal.
Image Credit: CYTENA GmbH
Start sorting right away
Separating cells is easy and contamination-free with disposable cartridges. Users can just pipette their sample into the reservoir and fix the cartridge to the B.SIGHT. Microscopic droplets are generated through the bacteria dispensing cartridges to make stable and precise bacteria encapsulation.
Isolate fluorescent microorganisms
CYTENA provides customers the option to add one combined laser for fluorescent cell sorting. Users can select the laser that best suits their requirements and start classifying immediately.
Image Credit: CYTENA GmbH
Intuitive and easy to use software
Users can make use of the interactive well plate to design their special dispensing experiment and observe in real-time how every bacterium is grouped via the cartridge’s nozzle and dispensed into their 96- or 384-well plate. Images of the dispensed single bacterium have been stored on the hard drive, thereby offering proof of clonality.
Image Credit: CYTENA GmbH
Microbiology research made easy
- Automation is all set
- High-resolution optics is offered
- Consistent with anaerobic conditions
Isolate more species with the B.SIGHT
List of pure cultures isolated with the B.SIGHT
- Staphylococcus aureus
- Bacillus subtilis
- Enterococcus faecalis
- Bacillus vulgatus (strict anaerobe)
- Escherichia coli (Top10, DH5alpha, BL21, etc.)
- Various cyanobacteria strains
- Pichia pastoris (yeast)
- Saccharomyces cerevisiae (yeast)
List of complex samples processed with the B.SIGHT
- Wastewater
- Mouse caecal samples
- Human fecal samples
- Human oral microbiome
- Marine samples
Control the device from outside the anaerobic bench
Users can connect the monitor, keyboard, and mouse or even use a remote control terminal
Video Credit: CYTENA GmbH
Applications
- Accelerating bacteria transformation and cloning workflows using microbial single-cell isolation
- High-throughput cultivation workflow for isolation of anaerobic gut bacteria
- Sequencing single-cell genomes of uncultivated bacteria in the human oral microbiome