Overview
The high-throughput microbial isolation platform, B.Sight™, is available for anaerobic cultivation, genomics, and strain development.
The second generation of B.SIGHT is known to be a microbial isolation platform that makes use of advanced optical microscopy and single-cell dispensing technology to allow the separation of the smallest microbial cells.
This device tends to adapt microbiology research by enabling quick and effective separation of bacteria from complicated microbiome samples or converted yeast and/or bacteria cultures that have been utilized for strain development.
Features
- Rapid high-throughput single-cell isolation and cloning of microorganisms can be done
- Guarantee of clonality
- Separate single bacteria in a 384-well plate in just 8 minutes
- Friendly automation
- Accurate deposition of single cells onto PCR plates
- Completely operational within the anaerobic cabinets
Brightfield and fluorescence
- Bright-field and fluorescent sorting can be done
- Low-pressure system reduces cell stress
- Single-use cartridge needs no cleaning, thereby decreasing the risk of cross-contamination
- Easy and rapid operation, isolation into a 384-well plate in less than 8 minutes
Eliminate cross contamination
Gentle dispensing
- E. coli cell viability >90%
- Yeast viability >98%
EASY.ON design
- Simple loading
- Perfect for anaerobic cabinets
Precise single-cell dispensing into PCR plates
- Automated Offset Correction guarantees accurate and reproducible cell deposition at the center of every well (96/384)
- Customized for single-cell genomics for uncultured microbiota
- Allows downstream miniaturization of cell lysis in sub-microliter volumes (<1 µl)
Image Credit: CYTENA GmbH
Assurance of single-cell dispensing
- Real-time imaging and processing of single-cell dispensing can be done
- Trustworthy single-cell cloning of yeast and bacteria
- Nozzle images documented for each isolation event
Image Credit: CYTENA GmbH
Unprecedented dispensing speed
The separation of microorganisms into multi-well plates has been done in just minutes. B.SIGHT could be dispensed into single, 96- or 384- microwell low-profile deep well plates.
Image Credit: CYTENA GmbH
Processing times
Source: CYTENA GmbH
| |
Time |
| System boot until experiment starts |
<5 min |
| Dispensing (96-well plate) single cells |
~3 min |
| Dispensing (384-well plate) single cells |
~8 min |
| Shutdown and cleaning time |
<5 min |
Integrate into users’ automated workflows
The B.SIGHT could be easily combined into automated strain development and microbiome workflows.
Once dispensing is done, B.SIGHT’s lid automatically opens enabling robotic arms to shift the plate downstream for additional processing.
Applications
Image Credit: CYTENA GmbH
Accelerating bacteria transformation and cloning workflows using microbial single-cell isolation
Image Credit: CYTENA GmbH
High-throughput cultivation workflow for isolation of anaerobic gut bacteria
Image Credit: CYTENA GmbH
Sequencing single-cell genomes of uncultivated bacteria in the human oral microbiome