Overview
Cryopreserved primary Rabbit hepatocytes are co-cultured with non-parenchymal stromal cells in HUREL® Rabbit™.
Example Metabolic Activity (nmoles/hr/106 cells). Source: Visikol Inc.
| Substrate |
Enzyme |
Concentration (µM) |
Day 1 |
Day 4 |
Day 8 |
| 7-Ethoxycoumarin |
Phase I (ECOD) |
100 |
3.35 |
2.74 |
.94 |
| 7-Hydroxycoumarin |
Phase II (UGT) |
100 |
295.33 |
278.50 |
265.33 |
| 7-Hydroxycoumarin |
Phase II(Sult) |
100 |
48.17 |
37.83 |
30.65 |
Thawed and plated with 10% serum-supplemented HUREL PlatinumHeps™ Media, cryopreserved rabbit hepatocytes were switched to HUREL PlatinumHeps™ maintenance Media 24 hours after seeding.
The CYP substrate concentrations and metabolite formation, which is expressed as nmoles/hr/million cells, are listed in the table above. On Days 1, 4, and 8 (the customer day), all incubations were performed in triplicate and incubated for 60 minutes.
At 37 °C and with 5% CO2, reactions took place in a humid incubator. Before undergoing further LC/MS/MS analysis, collected supernatants were kept at –20 °C.
Phase contrast image in a 24-well at a 10× magnification. Image Credit: Visikol Inc.
Phase contrast image in a 24-well at a 10× magnification. Image Credit: Visikol Inc.
Bile canaliculi assayed via 5-(and-6)-carboxy-2’, 7’-dichlorofluorescein diacetate (CDFDA) stain at a concentration of 5 µM and imaged in the GFP channel in a 24-well at 10× magnification. Image Credit: Visikol Inc.
Example culture origin
Animal donor demographics
- Strain: New Zealand White
- Number of Donors: 4
- Age: –
- Gender: Male