Overview

HUREL® Rat SD™ is a co-culture made up of non-parenchymal stromal cells and cryopreserved primary rat hepatocytes.

Example Metabolic Activity (nmoles/hr/106 cells). Source: Visikol Inc.

Substrate Enzyme Concentration (µM) Day 1 Day 4 Day 8
7-Ethoxycoumarin Phase I 100 0.074 0.183 0.181
7-Hydroxycoumarin Phase II 100 14.380 6.640 8.460
7-Hydroxycoumarin Phase II 100 3.848 2.484 1.572

 

Primary rat hepatocytes that had been cryopreserved were thawed, plated on HUREL PlatinumHeps™ Media supplemented with 10% serum, and then switched to HUREL PlatinumHeps™ maintenance Media 24 hours later.

The concentrations of CYP substrates are listed in the table above, and metabolite formation is expressed as nmoles/hr/106 cells. On days D1, D4, and D8, after cell delivery, all incubations were performed in triplicate and incubated for 60 minutes.

The reactions happened in a humid incubator with 5% CO2 at 37 °C. Until a subsequent LC/MS/MS analysis, collected supernatants were kept at –20 °C.

  • Day 1-Morphology

HUREL® Rat SD™

Phase contrast image in a 24-well at a 10× magnification. Image Credit: Visikol Inc.

  • Day 7-Morphology

HUREL® Rat SD™

Phase contrast image in a 24-well at a 10× magnification. Image Credit: Visikol Inc.

  • Day 7-Bile Canaliculi

HUREL® Rat SD™

Bile canaliculi assayed via 5- (and-6)-carboxy-2’, 7’- dichlorofluorescein diacetate (C- DCFDA) stain at a concentration of 5 μM and imaged in the GFP channel in a 96-well at 10× magnification with filters EX/EM 492-495/512-527 nm. Image Credit: Visikol Inc.

Example culture origin

Rat donor demographics

  • Strain: Sprague-Dawley
  • Number of Donors: 16
  • Age: 2 months
  • Gender: Male

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