HUREL® Rat WH™

Overview

Co-culture made of cryopreserved primary rat hepatocytes cultured with cells of non-parenchymal, stromal type.

Source: Visikol, Inc.

Features

Culture conditions and morphology

Cryopreserved human hepatocytes were defrosted and plated with the help of the HUREL PlatinumHeps™ Media supplemented with 10% serum and consequently changed 24 hours post-seeding to the HUREL PlatinumHeps™ Basal Media.

In the table, CYP substrate concentrations are provided above in the table together with metabolite formation recorded as nmoles/hr/106 cells. All incubations were performed in triplicate on Days 1, 4, and 8 upon cell delivery and incubated for around 60 minutes.

Reactions occur in a humidified incubator ranging at 37 °C, in 5% CO₂. Supernatants were gathered and stored at –20 °C, until the following LC/MS/MS analysis.

• Day 1–Morphology

Phase contrast image in a 24-well at a 10× magnification. Image Credit: Visikol, Inc.

• Day 7–Morphology

Phase contrast image in a 24-well at a 10× magnification. Image Credit: Visikol, Inc.

• Day 7–Bile Canaliculi

Bile canaliculi assayed via 5-(and-6)-carboxy-2′, 7′ – dichlorofluorescein diacetate (C-DCFDA) stain at a concentration of 5 microns and imaged in the GFP channel in a 96-well at 10× magnification with filters EX/EM 492-495/512-527 nm. Image Credit: Visikol, Inc.

Example culture origin

Human donor demographics

• Gender: Male
• Age (years): 65
• Race: Caucasian
• Cause of death: IHC-Stroke
• Drug history: Not provided
• Medications: Unidentified statin, Lisinopril, Lipitor, Clonaidine, and Multivitamin
• Medical history: Basel cell: 15 to 20 years ago. Eliminated with no radiation-received no chemo or radiation with no reoccurrence. Around 20 to 25 years ago, hypertension was diagnosed.

Donor serology

• Hepatitis B: Negative
• Hepatitis C: Negative
• EBV: IgG [+], IgM [-}
• HIV: Negative
• CMV: Positive
• RPR: Negative