Overview
Tissues can now be quickly and effectively imaged in three dimensions without impacting the underlying cellular morphology using the Visikol HISTO tissue-clearing method.
The Visikol HISTO approach, which is reversible and designed to work in conjunction with immunolabeling, has been developed to enable the continuation of 3D histology with traditional 2D histology.
Visikol HISTO-1 and Visikol HISTO-2 are two different reagents used in the Visikol HISTO tissue-clearing procedure. First Visikol HISTO-1 is used, then Visikol HISTO-2.
For small tissues, such as thin brain slices (500 μm), only Visikol HISTO-1 is required, and Visikol HISTO-1 is capable of imaging tissues. Larger tissues must first clear in Visikol HISTO-2 before being inserted there to image larger samples.
It takes approximately 7 mL of Visikol HISTO-2, which has a refractive index of 1.53, to cover the entire mouse brain.