Overview
Advanced BioMatrix’s Collagen Hybridizing Peptides (CHP) conjugates are labeled with biotin (B-CHP) for avidin/streptavidin-mediated detection or with 5-FAM (F-CHP)/sulfo-Cyanine3 (R-CHP) for fluorescence detection.
The distinctive protein structure of collagen is the triple helix. The triple helical collagen molecules are broken down by particular proteases (like MMP or cathepsin K) during tissue remodeling and become unfolded at body temperature.
A synthetic peptide called the Collagen Hybridizing Peptide (CHP) has the ability to specifically bind to these denatured collagen strands through hydrogen bonding in histology, in vivo, and in vitro (3D cell culture).
CHP has a strong ability to hybridize with denatured collagen strands because it shares the structural motif and the Gly-X-Y repeating sequence with natural collagen. This process is comparable to a DNA fragment annealing to its complementary DNA strand during PCR.
Due to the lack of binding sites and the fact that it is neutral and hydrophilic, CHP is a highly specific probe for unfolded collagen molecules. It also has little affinity for intact collagen molecules and is inert to non-specific binding.
Source: Advanced BioMatrix
| Parameter, Testing, and Method |
CHP 5-FAM #5264 |
| Specialty |
Straight-forward fluore-scence detection in green |
| Formula |
C135H175N31O45 |
| Molecular Weight |
2952.01 g/mol |
| Ex/Em |
494 nm / 512 nm |
| Synonym |
Collagen Mimetic Peptide, CMP |
| Solubility |
Aqueous Buffers |
| Storage |
-20 °C as powder for long term storage. 4 °C after reconstitution in water. Protect from light. |
| Source |
Synthetic |
| Shelf Life |
Minimum of 6 months from date of receipt |
Storage/Stability
The product is shipped in ambient conditions. The product should be kept at –20 °C
Features
- Appropriate for both frozen and paraffin-embedded sections without the requirement of antigen retrieval
- More informative, reliable and convenient than zymography, DQ collagen, SHG, and TEM
- Applicable to denatured collagens of all subtypes and from all species; binding relying on collagen’s secondary structure instead of specific epitopes
- Stable in solution under 4 °C, eliminating the need to aliquot for storage
- A non-antibody approach with no species restrictions, compatible for co-staining with any antibody
- High affinity and unparalleled specificity to collagen with essentially no nonspecific binding
- Small size (2% of IgG by MW) enabling facile tissue penetration during whole tissue staining (with no need for sectioning)