Lifeink® 220 from Advanced BioMatrix is a 70 mg/ml type I collagen bioink that has been developed for extrusion-based FRESH 3D bioprinting. Lifeink® 220 is available as a pH neutral, isotonic bioink, and is all set for bioprinting and cell addition.

For printing to be done with Lifeink® 220, Advanced BioMatrix highly suggests making use of the FRESH printing method and printing at 2 to 8 °C. Three-dimensional (3D) gels enable the study of the effects of the mechanical properties of the extracellular matrix (ECM), like rigidity and density, on migration, cell development, and morphology.

Diverse 2D systems, 3D environments enable cell extensions to concurrently communicate with integrins on all cell surfaces, leading to the triggering of particular signaling pathways. Also, gel rigidity or stiffness impacts cell migration otherwise in 3D versus 2D surroundings.

A bioink that matches life must consist of the following traits: High Print Resolution, strong mechanical strength, shear-thinning, excellent cytocompatibility, enable for cellular remodeling and biomimetic. Lifeink® 220 satisfies those needs.

Advanced BioMatrix provides Lifeink® 220, a bioink that is a 70 mg/ml type I collagen. Lifeink® 220 is pH-neutral collagen along with physiological salt concentration. The starting material is purified with the help of a composing manufacturing process.

Lifeink® 220 bioink has the following characteristics as displayed below:

Lifeink® 220

Image Credit: Advanced BioMatrix

Source: Advanced BioMatrix

Parameter, Testing, and Method Lifeink® 220 #5343
Sterilization Method Filtration, Aseptic Processing
Sterility - USP modified No growth
Form Opaque Gel/Paste
Package Size 4 mL
Storage Temperature 2-8 °C
Print Temperature 2-8 °C
Shelf Life Minimum of 6 months from date of receipt
Endotoxin - LAL ≤ 10.0 EU/mL
pH 6.9-7.6
Osmolality (mOsmo H2O/kg) 290-370
Source Material Bovine Collagen
Electrophoretic Pattern - Coomassie Blue Characteristic
Collagen Concentration - Biuret 65-75 mg/mL
Collagen Purity - Silver Staining ≥ 99%
Continuous Flow Extrusion Pass

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