Overview
A special solution developed for 2D coatings or 3D hydrogels is TeloCol-6, which is 50 mL of 6 mg/ml, type I bovine telocollagen.
This kit is available with a neutralization solution for simple handling and preparation. Being a higher concentration telocollagen, TeloCol-6 will polymerize rapidly and develop powerful hydrogels compared to atelocollagen.
TeloCol®-6 bovine collagen solution has been acquired from an acid extraction process providing telopeptide-intact collagen. TeloCol® seems to be soluble telocollagen in 0.01 N HCL. The telopeptide regions present at both ends of the collagen chain, N- and C-telopeptide regions, are retained.
Approximately, TeloCol®-6 collagen is 95% Type I collagen with the leftover consisting of Type III collagen. Every product contains a bottle comprising 50 ml of collagen solution joined with a bottle of pre-formulated neutralizing solution for the development of a collagen gel.
This product has been delivered at a concentration of nearly 6 mg/ml. TeloCol®-6 is sterile filtered and offered in easy-to-use packaging for use and storage.
TeloCol®-6 is perfect for coating surfaces, offering preparation of thin layers for culturing cells, or using as a solid gel.
Image Credit: Advanced Biomatrix
Source: Advanced Biomatrix
| Parameter, Testing, and Method |
TeloCol®-6 Type I Collagen #5225 |
| Sterilization Method |
Filtration |
| Extraction Method |
Acid - telocollagen |
| Form |
Solution |
| Package Size |
50 mL Kit |
| Storage Temperature of Collagen |
2-10 °C |
| Storage Temperature of Neutralization Solution |
Room Temperature |
| Shelf Life |
Minimum of 6 months from date of receipt |
| Collagen Concentration - Biuret |
5.8-6.8 mg/mL |
| Collagen Purity - Silver Staining |
≥ 99% |
| pH |
1.9-3.0 |
| Kinetic Gel Test (Minutes) |
≤ 40 |
| Gel Formation Tube Test (Minutes) |
≤ 40 |
| Fibrillogenesis (Absorbance Units) |
≥ 0.35 |
| Electrophoretic Pattern - Coomassie Blue |
Characteristic |
| Sterility - USP modified |
No growth |
| Endotoxin - LAL |
≤ 10.0 EU/mL |
| Osmolality (mOsmo H2O/kg) |
≤ 35 |
| Cell Attachment Assay |
Pass |
| Source |
Bovine Hide |
| Hydrogel Young's Modulus E (Pa) |
Characteristic |