Overview
With the help of a multi-step process, this Type IV Collagen has been separated from the human placenta and has been purified. The product is supplied as an ascetic, lyophilized powder comprising 5 mg of Type IV collagen per vial.
This Type IV Collagen is detached from the human placenta and has been purified with the help of a multi-step process. The product is supplied as a sterile, lyophilized powder containing 5 mg of Type IV collagen per vial.
Type IV Collagen is considered to be the primary collagen discovered in the extracellular basement membranes isolating a range of endothelial and epithelial cells. It is a significant component of the dermal-epidermal junction where it is majorly discovered in the lamina densa. It is a heterotrimeric molecule comprising two Alpha 1-like and one Alpha 2-like chain.
Normally, Type IV Collagen is utilized as a thin coating on tissue culture surfaces. Particular instructions are found in the how-to-use section. This product is generally utilized in vitro as a substrate scaffold to improve cell adherence, attachment, and proliferation.
Type IV collagen might be utilized to culture epithelial, muscle, endothelial, nerve, and several other cell types. Besides, this product is ideal for use as a substrate for positive controls and collagenase assays.
Source: Advanced Biomatrix
| Parameter, Testing, and Method |
Type IV Collagen #5022 |
| Sterilization Method |
Irradiation |
| Form |
Lyophilized Powder |
| Package Size |
5 mg |
| Storage Temperature |
-20 °C prior to reconstitution and 2-10 °C after reconstitution |
| Shelf Life |
Minimum of 6 months from date of receipt |
| Shelf Life after Reconstitution |
3 months |
| Adventitious Agents |
Human source has been tested and found negative for Hepatitis B, C and immunodeficiency virus-1. |
| Electrophoretic Pattern - Coomassie Blue |
Characteristic |
| Sterility - USP modified |
No growth |
| Endotoxin - LAL |
≤ 1.0 EU/mL |
| Cell Attachment Assay |
Pass |
| Source |
Human Placenta |