Overview
Lyophilized methacrylated Type I collagen in the amount of 100 mg is called PhotoCol®. PhotoCol® offers native-like 3D collagen gels that may be created at varied concentrations and photocrosslinked (needs a photoinitiator) to deliver varying gel stiffness.
PhotoCol® kits comprise purified methacrylated Type I bovine collagen as the core component along with other support reagents present in the kit. This PhotoCol® product just consists of methacrylate collagen. The kits each consist of the first three rows displayed below and come with the chosen photoinitiator.
Image Credit: Advanced BioMatrix
Table 1. Source: Advanced BioMatrix
| Item |
Catalog Number |
Package Size |
Storage Temperature |
| Methacrylated Collagen |
#5198 |
100 mg |
2-10 °C |
| 20 mM Acetic Acid |
#5079 |
50 mL |
2-30 °C |
| Neutralization Solution |
#5205 |
10 mL |
Room Temperature |
The methacrylated Type I collagen has been produced from telo-peptide intact bovine collagen where the collagen has been subjected to alteration by reacting with the free amines.
This is mainly the ε-amines groups of the lysine residues as well as the a-amines groups on the N-termini. Almost, >20% of the total lysine residues of the collagen molecule have been methacrylated. The collagen has been withdrawn from the bovine hide and consists of a high monomer content.
Table 2. Source: Advanced BioMatrix
| Parameter, Testing, and Method |
Methacrylated Collagen #5198 |
| Sterilization Method |
Filtration |
| Extraction Method |
Acid - Telocollagen |
| Form |
Lyophilized Powder |
| Degree of Methacrylation |
≥ 20% |
| Package Size |
100 mg |
| Storage Temperature |
2-10 °C |
| Shelf Life |
Minimum of 6 months from date of receipt |
| Shelf Life After Reconstitution |
2 months |
| Collagen Purity - Silver Staining |
≥ 99% |
| Kinetic Gel Test (Minutes) |
≤ 40 |
| Gel Formation Tube Test (Minutes) |
≤ 40 |
| Electrophoretic Pattern - Coomassie Blue |
Characteristic |
| Sterility - USP modified |
No growth |
| Endotoxin (LAL) |
≤ 10.0 EU/mL |
| Source |
Bovine Hide |
| Hydrogel Young's Modulus E (Pa) |
Characteristic |