Overview
For 2D and 3D cell culture, FibriCol® is a solution of type I bovine atelocollagen at 10 mg/ml. FibriCol® hydrogels are firmer than products with lower concentrations of collagen due to their high concentration.
FibriCol® collagen is regarded as the gold standard among all collagens for functionality, purity (>99% collagen content), and the most natural-like collagen on the market. Bovine hides from the only regulated, closed herd in the US are isolated for FibriCol®.
The manufacturing procedures used by Advanced BioMatrix adhere to demanding quality standards, which have produced products with unmatched lot-to-lot consistency.
About 97% of the collagen in FibriCol® is Type I collagen, with the remaining 2% being Type III collagen. FibriCol® collagen is supplied at a concentration of roughly 10 mg/mL.
On a Certificate of Analysis that is provided with the purchase of each product, the concentration for each particular lot is listed. Since FibriCol® is soluble in 0.01 N HCl and atelocollagen, the pH is 2.
When high collagen concentrations are required, FibriCol® collagen is the best choice. For use and storage, FibriCol® collagen comes in a 20 mL volume and is housed in convenient packaging. FibriCol® is provided as a ready-to-use solution and is sterile filtered.
Source: Advanced BioMatrix
| Parameter, Testing, and Method |
FibriCol® Type I Collagen #5133 |
| Sterilization Method |
Filtration |
| Extraction Method |
Enzyme - atelocollagen |
| Form |
Solution |
| Package Size |
20 mL, 100 mL |
| Storage Temperature |
2-10 °C |
| Shelf Life |
Minimum of 6 months from date of receipt |
| Collagen Concentration - Biuret |
9.0-11.5 mg/mL |
| Collagen Purity - Silver Staining |
≥ 99.9% |
| pH |
1.9-2.1 |
| Kinetic Gel Test (Minutes) |
≤ 40 |
| Gel Formation Tube Test (Minutes) |
≤ 40 |
| Fibrillogenesis (Absorbance Units) |
≥ 0.5 |
| Electrophoretic Pattern - Coomassie Blue |
Characteristic |
| Sterility - USP modified |
No growth |
| Endotoxin - LAL |
≤ 1.0 EU/mL |
| Osmolality (mOsmo H2O/kg) |
≤ 35 |
| Cell Attachment Assay |
Pass |
| Source |
Bovine Hide |
| Hydrogel Young's Modulus E (Pa) |
Characteristic |