For 2D and 3D cell culture, FibriCol® is a solution of type I bovine atelocollagen at 10 mg/ml. FibriCol® hydrogels are firmer than products with lower concentrations of collagen due to their high concentration.

FibriCol® collagen is regarded as the gold standard among all collagens for functionality, purity (>99% collagen content), and the most natural-like collagen on the market. Bovine hides from the only regulated, closed herd in the US are isolated for FibriCol®.

The manufacturing procedures used by Advanced BioMatrix adhere to demanding quality standards, which have produced products with unmatched lot-to-lot consistency.

About 97% of the collagen in FibriCol® is Type I collagen, with the remaining 2% being Type III collagen. FibriCol® collagen is supplied at a concentration of roughly 10 mg/mL.

On a Certificate of Analysis that is provided with the purchase of each product, the concentration for each particular lot is listed. Since FibriCol® is soluble in 0.01 N HCl and atelocollagen, the pH is 2.

When high collagen concentrations are required, FibriCol® collagen is the best choice. For use and storage, FibriCol® collagen comes in a 20 mL volume and is housed in convenient packaging. FibriCol® is provided as a ready-to-use solution and is sterile filtered.

Source: Advanced BioMatrix

Parameter, Testing, and Method FibriCol® Type I Collagen #5133
Sterilization Method Filtration
Extraction Method Enzyme - atelocollagen
Form Solution
Package Size 20 mL, 100 mL
Storage Temperature 2-10 °C
Shelf Life Minimum of 6 months from date of receipt
Collagen Concentration - Biuret 9.0-11.5 mg/mL
Collagen Purity - Silver Staining ≥ 99.9%
pH 1.9-2.1
Kinetic Gel Test (Minutes) ≤ 40
Gel Formation Tube Test (Minutes) ≤ 40
Fibrillogenesis (Absorbance Units) ≥ 0.5
Electrophoretic Pattern - Coomassie Blue Characteristic
Sterility - USP modified No growth
Endotoxin - LAL ≤ 1.0 EU/mL
Osmolality (mOsmo H2O/kg) ≤ 35
Cell Attachment Assay Pass
Source Bovine Hide
Hydrogel Young's Modulus E (Pa) Characteristic

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