Overview
RatCol® for 3D gels is 25 mL of 4 mg/ml, type I rat tail telocollagen solution available for 3D hydrogels or 2D coatings.
This kit for 3D gels is available with a neutralization solution for simple handling. Being telocollagen, RatCol® available for 3D gels will develop harder hydrogels compared to atelocollagen.
RatCol® Type I Acid Soluble Rat Tail Collagen consists of 100 mg at a concentration of nearly 4 mg/mL in a 0.02M acetic acid solution (~pH 3). RatCol® collagen seems to be soluble telo-collagen.
Every product consists of a bottle including 100 mg of collagen solution equipped with a bottle of pre-formulated neutralizing solution for the development of a collagen gel. This collagen product has been offered in easy-to-use packaging for use and storage. This product is sterile-filtered and is supplied as a user-friendly solution.
This product is perfect for coating surfaces, offering the generation of thin layers for culturing cells, or using as a solid gel. RatCol® collagen is appropriate for applications with the help of a range of cell lines such as fibroblasts, hepatocytes, and epithelial cells.
Source: Advanced Biomatrix
| Parameter, Testing, and Method |
RatCol® Type I Collagen #5153 |
| Sterilization Method |
Filtration |
| Extraction Method |
Acid - telocollagen |
| Form |
Solution |
| Package Size |
100 mg (~25 mL) Kit |
| Storage Temperature of Collagen |
2-10 °C |
| Storage Temperature of Neutralization Solution |
Room Temperature |
| Shelf Life |
Minimum of 6 months from date of receipt |
| Collagen Concentration - Biuret |
3.5-4.5 mg/mL |
| Collagen Purity - Silver Staining |
≥ 99% |
| pH |
3.0-3.8 |
| Kinetic Gel Test (Minutes) |
≤ 40 |
| Gel Formation Tube Test (Minutes) |
≤ 40 |
| Electrophoretic Pattern - Coomassie Blue |
Characteristic |
| Sterility - USP modified |
No growth |
| Endotoxin - LAL |
≤ 10.0 EU/mL |
| Osmolality (mOsmo H2O/kg) |
≤ 35 |
| Cell Attachment Assay |
Pass |
| Source |
Rat Tail Tendon |
| Hydrogel Young's Modulus E (Pa) |
Characteristic |