Overview
For 2D and 3D cell culture, or as a collagen standard, PureCol® is a solution of Type I bovine atelocollagen at a concentration of 3 mg/ml. With a ≥99.9% collagen content and citations in more than 2000 publications, PureCol® is the benchmark among collagen products.
PureCol® collagen is regarded as the gold standard among collagens for purity (≥99.9% collagen content), usability, and being the closest to native collagen possible. Bovine hides from the only controlled, closed herd in the US are isolated for PureCol®.
The manufacturing procedures used by Advanced BioMatrix adhere to exacting quality standards, which have been shown to produce unmatched consistency from lot to lot.
About 97% of the collagen in PureCol® is Type I atelocollagen, with the remaining 3% being Type III collagen. The concentration of PureCol® collagen is around 3 mg/ml. On a Certificate of Analysis that is provided with the purchase of each product, the concentration for each particular lot is listed.
Since atelocollagen in PureCol® is soluble in 0.01 N HCl, the pH is 2. PureCol® collagen is excellent for coating surfaces, preparing thin layers for cell culture, or using it as a solid gel.
The user-friendly packaging for PureCol® collagen makes it easy to use and store. Filtered and sterile, PureCol® is provided as a ready-to-use solution.
Source: Advanced BioMatrix
| Parameter, Testing, and Method |
PureCol® Type I Collagen #5005 |
| Sterilization Method |
Filtration |
| Extraction Method |
Enzyme - atelocollagen |
| Form |
Solution |
| Package Size |
100 mL, 1000 mL |
| Storage Temperature |
2-10 °C |
| Shelf Life |
Minimum of 6 months from date of receipt |
| Collagen Concentration - Biuret |
2.9-3.2 mg/mL |
| Collagen Purity - Silver Staining |
≥ 99.9% |
| pH |
1.9-2.1 |
| Kinetic Gel Test (Minutes) |
≤ 40 |
| Gel Formation Tube Test (Minutes) |
≤ 40 |
| Fibrillogenesis (Absorbance Units) |
≥ 0.5 |
| Electrophoretic Pattern - Coomassie Blue |
Characteristic |
| Sterility - USP modified |
No growth |
| Endotoxin - LAL |
≤ 1.0 EU/mL |
| Osmolality (mOsmo H2O/kg) |
≤ 35 |
| Cell Attachment Assay |
Pass |
| Source |
Bovine Hide |
| Hydrogel Young's Modulus E (Pa) |
Characteristic |