As a collagen standard, PureCol®-S is a solution of type I bovine atelocollagen at a concentration of 3 mg/ml. With >2000 publications citing it, PureCol®-S is recognized as the gold standard among collagen products for consistency, reproducibility, and purity (>99.9% collagen content).

When ultra-pure collagen is required for an assay, PureCol®-S collagen is offered as a standard. About 97% of the collagen in the PureCol®-S collagen standard is Type I collagen, and the remaining 2% is Type III collagen. According to the results of gel permeation chromatography, it has a high monomer content.

This product is given out at a concentration of about 3 mg/ml. By using the Biuret protein determination assay, the concentration is verified. The concentration for each specific lot is supplied with a Certificate of Analysis that is available with the purchase of each product. Since atelo-collagen in PureCol®-S is soluble in 0.01 N HCI, the pH is close to 2.0.

PureCol®-S is the best collagen standard to use in controlled testing and assay procedures. PureCol®-S is provided as a ready-to-use solution and has undergone sterile filtration.

Source: Advanced BioMatrix

Parameter, Testing, and Method PureCol®-S Collagen Standard #5015
Sterilization Method Filtration
Extraction Method Enzyme - atelocollagen
Form Solution
Package Size 20 mL
Storage Temperature 2-10 °C
Shelf Life Minimum of 6 months from date of receipt
Collagen Concentration - Biuret 2.9-3.2 mg/mL
Collagen Concentration - AAA 2.9-3.2 mg/mL
Collagen Purity - Silver Staining ≥ 99.9%
pH 1.9-2.1
Kinetic Gel Test (Minutes) ≤ 40
Gel Formation Tube Test (Minutes) ≤ 40
Fibrillogenesis (Absorbance Units) ≥ 0.5
Electrophoretic Pattern - Coomassie Blue Characteristic
Sterility - USP modified No growth
Endotoxin -LAL ≤ 1.0 EU/mL
Osmolality (mOsmo H2O/kg) ≤ 35
Source Bovine Hide
Hydrogel Young's Modulus E (Pa) Characteristic

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