Overview
As a collagen standard, PureCol®-S is a solution of type I bovine atelocollagen at a concentration of 3 mg/ml. With >2000 publications citing it, PureCol®-S is recognized as the gold standard among collagen products for consistency, reproducibility, and purity (>99.9% collagen content).
When ultra-pure collagen is required for an assay, PureCol®-S collagen is offered as a standard. About 97% of the collagen in the PureCol®-S collagen standard is Type I collagen, and the remaining 2% is Type III collagen. According to the results of gel permeation chromatography, it has a high monomer content.
This product is given out at a concentration of about 3 mg/ml. By using the Biuret protein determination assay, the concentration is verified. The concentration for each specific lot is supplied with a Certificate of Analysis that is available with the purchase of each product. Since atelo-collagen in PureCol®-S is soluble in 0.01 N HCI, the pH is close to 2.0.
PureCol®-S is the best collagen standard to use in controlled testing and assay procedures. PureCol®-S is provided as a ready-to-use solution and has undergone sterile filtration.
Source: Advanced BioMatrix
| Parameter, Testing, and Method |
PureCol®-S Collagen Standard #5015 |
| Sterilization Method |
Filtration |
| Extraction Method |
Enzyme - atelocollagen |
| Form |
Solution |
| Package Size |
20 mL |
| Storage Temperature |
2-10 °C |
| Shelf Life |
Minimum of 6 months from date of receipt |
| Collagen Concentration - Biuret |
2.9-3.2 mg/mL |
| Collagen Concentration - AAA |
2.9-3.2 mg/mL |
| Collagen Purity - Silver Staining |
≥ 99.9% |
| pH |
1.9-2.1 |
| Kinetic Gel Test (Minutes) |
≤ 40 |
| Gel Formation Tube Test (Minutes) |
≤ 40 |
| Fibrillogenesis (Absorbance Units) |
≥ 0.5 |
| Electrophoretic Pattern - Coomassie Blue |
Characteristic |
| Sterility - USP modified |
No growth |
| Endotoxin -LAL |
≤ 1.0 EU/mL |
| Osmolality (mOsmo H2O/kg) |
≤ 35 |
| Source |
Bovine Hide |
| Hydrogel Young's Modulus E (Pa) |
Characteristic |